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- Antibody Data
- Antigen structure
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- Immunohistochemistry [3]
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- Product number
- MA5-15383 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRYAB Monoclonal Antibody (1D11C6E6)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15383 targets CRYAB in indirect ELISA, IHC and WB applications and shows reactivity with Human samples. The MA5-15383 immunogen is purified recombinant fragment of CRYAB (aa1-175) expressed in E. Coli.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1D11C6E6
- Vial size
- 100 μL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Presence of melanopsin in human crystalline lens epithelial cells and its role in melatonin synthesis.
Alkozi HA, Wang X, Perez de Lara MJ, Pintor J
Experimental eye research 2017 Jan;154:168-176
Experimental eye research 2017 Jan;154:168-176
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissues using CRYAB monoclonal antibody (Product # MA5-15383).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human brain tumor tissue sections. To expose target antigens, heat-induced epitope retrieval was performed by heating tissues at 120°C for 5 minutes in sodium citrate buffer pH 6.0. Tissues were blocked in 3% hydrogen peroxide for 5 minutes at room temperature and probed with a CRYAB monoclonal antibody (Product # MA5-15383) at a dilution of 1:500 for 80 minutes at room temperature. Tissues were washed extensively with TBST. Detection was performed using an HRP-conjugated anti-mouse IgG secondary antibody and colorimetric substrate. Tissues were counterstained with hematoxylin and visualized by light microscopy. Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human brain hippocampus (A), lung cancer (B), brain tumor (C), breast cance (D) using CRYAB monoclonal antibody (Product # MA5-15383) followed with DAB staining.
