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- Antibody Data
- Antigen structure
- References [4]
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- Western blot [1]
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- Product number
- PA1-011 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-CRYAB (Ser45) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-011 detects phospho-alpha-B crystallin (Ser45) from bovine, human, mouse and rat samples. This antibody does not detect the unphosphorylated form of the protein. PA1-011 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~20 kDa protein representing recombinant bovine phospho-alpha-B crystallin (Ser45). Immunofluorescence staining of phospho-alpha-B crystallin (Ser45) in U373 MG cells results in cytoplasmic staining. The PA1-011 immunogen is a synthetic phopshopeptide corresponding to residues L(44) S(p) P F Y L R P P S F(54) C of human alpha-B Crystallin. Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Phosphorylation of αB-crystallin supports reactive astrogliosis in demyelination.
Hyperglycemia induced expression, phosphorylation, and translocation of αB-crystallin in rat skeletal muscle.
Expression and induction of small heat shock proteins in rat heart under chronic hyperglycemic conditions.
O-GlcNAcylation of αB-crystallin regulates its stress-induced translocation and cytoprotection.
Kuipers HF, Yoon J, van Horssen J, Han MH, Bollyky PL, Palmer TD, Steinman L
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 28;114(9):E1745-E1754
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 28;114(9):E1745-E1754
Hyperglycemia induced expression, phosphorylation, and translocation of αB-crystallin in rat skeletal muscle.
Reddy VS, Jakhotia S, Reddy PY, Reddy GB
IUBMB life 2015 Apr;67(4):291-9
IUBMB life 2015 Apr;67(4):291-9
Expression and induction of small heat shock proteins in rat heart under chronic hyperglycemic conditions.
Reddy VS, Kumar ChU, Raghu G, Reddy GB
Archives of biochemistry and biophysics 2014 Sep 15;558:1-9
Archives of biochemistry and biophysics 2014 Sep 15;558:1-9
O-GlcNAcylation of αB-crystallin regulates its stress-induced translocation and cytoprotection.
Krishnamoorthy V, Donofrio AJ, Martin JL
Molecular and cellular biochemistry 2013 Jul;379(1-2):59-68
Molecular and cellular biochemistry 2013 Jul;379(1-2):59-68
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of recombinant bovine phospho-alpha-B crystallin (Ser45) using Product # PA1-011. Lane 1 is phospho-alpha-B crystallin (Ser45) and lane 2 is unphosphorylated alpha-B crystallin.
