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Learn200-301-F75
antibody from Rockland Immunochemicals, Inc.
Targeting: HSP90AA1
FLJ31884, Hsp89, Hsp90, HSP90N, HSPC1, HSPCA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 200-301-F75 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-F75, RRID:AB_2611181
- Product name
- Anti-HSP90?? (MOUSE) Monoclonal Antibody - 200-301-F75
- Antibody type
- Monoclonal
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of mouse anti-Hsp90 total antibody. Lane 1: A431. Lane 2: A549. Lane 3: HCT116. Lane 4: HeLa. Lane 5: HEK293. Lane 6: HepG2. Lane 7: HL-60. Lane 8: HUVEC. Lane 9: Jurkat. Lane 10: MCF7. Lane 11: PC3. Lane 12: T98G. Lane 13: Rat Brain. Load: 10 µg per lane. Primary antibody: Hsp90 total antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 84.7 kDa/~90for Hsp90 total. Other band(s): none.
- Validation comment
- Western Blot
