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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-51477 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCDC22 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: YQNFLYPSEP DLRDLLLFLA ERLPTDASED ADQPAGDSAI LLRAIGSQIR DQLALPWVPP HLRTPKLQHL QGSALQKPFH ASRLVVPELS SRGEPREFQA SPLLLPVPTQ VPQPVGRVA
- Concentration
- 0.3 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CCDC22 in Immunofluorescent staining of mouse piriform cortex shows neuronal staining in various layers. Samples were probed using a CCDC22 Polyclonal Antibody (Product # PA5-51477).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CCDC22 in Immunofluorescent staining of mouse parietal association cortex shows positivity in neuronal cell bodies and dendrites. Samples were probed using a CCDC22 Polyclonal Antibody (Product # PA5-51477).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Coiled-coil domain-containing protein 22 was achieved by transfecting NIH:OVCAR-3 with Coiled-coil domain-containing protein 22 specific siRNAs (Silencer® select Product # S226233, S26283). Western Blot analysis (Fig. a) was performed using Whole cell extracts from the Coiled-coil domain-containing protein 22 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with CCDC22 Polyclonal Antibody (Product # PA5-51477, 0.3 µg/mL ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this Western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Coiled-coil domain-containing protein 22.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-CCDC22 Polyclonal Antibody (Product # PA5-51477) and a 70 kDa band corresponding to Coiled-coil domain-containing protein 22 was observed across all the tested cell lines. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), NIH:OVCAR-3 (Lane 2), SK-O-V3 (Lane 3), U-2 OS (Lane 4), Mouse Prostate (Lane 5), Mouse Brain (Lane 6), Mouse Skeletal Muscle (Lane 7), Rat Prostate (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.3 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of CCDC22 in Immunofluorescent staining of mouse parietal association cortex shows distinct positivity in layer 4 neurons. Samples were probed using a CCDC22 Polyclonal Antibody (Product # PA5-51477).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of CCDC22 in human cell line A-431 using a CCDC22 Polyclonal Antibody (Product # PA5-51477) shows localization to nucleoplasm and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CCDC22 in Immunofluorescent staining of mouse olfactory bulb shows positivity in neurons and processes in the mitral and external plexiform layers. Samples were probed using a CCDC22 Polyclonal Antibody (Product # PA5-51477).
