11-0629-42
antibody from Invitrogen Antibodies
Targeting: SELL
CD62L, hLHRc, LAM-1, LAM1, Leu-8, LNHR, LSEL, Lyam-1, LYAM1, PLNHR
Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [6]
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- Product number
- 11-0629-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues. Applications Reported: The DREG-56 (DREG56) antibody has been reported for use in flow cytometric analysis. Applications Tested: The DREG-56 (DREG56) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- DREG-56 (DREG56)
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Induction of memory-like CD8+ T cells and CD4+ T cells from human naive T cells in culture.
Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.
Development of CAR-T cell therapy for B-ALL using a point-of-care approach.
Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.
n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.
CRISPR-Cas9-mediated disruption of PD-1 on human T cells for adoptive cellular therapies of EBV positive gastric cancer.
Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy.
Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans.
IL12-mediated sensitizing of T-cell receptor-dependent and -independent tumor cell killing.
CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients.
Common gamma chain cytokines promote rapid in vitro expansion of allo-specific human CD8+ suppressor T cells.
Human CD8+CXCR3+ T cells have the same function as murine CD8+CD122+ Treg.
Reciprocal CD4+ T-cell balance of effector CD62Llow CD4+ and CD62LhighCD25+ CD4+ regulatory T cells in small cell lung cancer reflects disease stage.
L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts.
Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule.
Tokumoto Y, Araki Y, Narizuka Y, Mizuno Y, Ohshima S, Mimura T
Clinical and experimental immunology 2022 Jan 28;207(1):95-103
Clinical and experimental immunology 2022 Jan 28;207(1):95-103
Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.
Genkel V, Dolgushin I, Baturina I, Savochkina A, Kuznetsova A, Pykhova L, Shaposhnik I
International journal of inflammation 2021;2021:6695468
International journal of inflammation 2021;2021:6695468
Development of CAR-T cell therapy for B-ALL using a point-of-care approach.
de Macedo Abdo L, Barros LRC, Saldanha Viegas M, Vieira Codeço Marques L, de Sousa Ferreira P, Chicaybam L, Bonamino MH
Oncoimmunology 2020;9(1):1752592
Oncoimmunology 2020;9(1):1752592
Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.
Yang C, Siebert JR, Burns R, Gerbec ZJ, Bonacci B, Rymaszewski A, Rau M, Riese MJ, Rao S, Carlson KS, Routes JM, Verbsky JW, Thakar MS, Malarkannan S
Nature communications 2019 Sep 2;10(1):3931
Nature communications 2019 Sep 2;10(1):3931
n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.
Wu B, Wang L, Jiang L, Dong L, Xu F, Lu Y, Jin J, Wang Z, Liang G, Shan X
Molecular medicine reports 2018 Jan;17(1):179-185
Molecular medicine reports 2018 Jan;17(1):179-185
CRISPR-Cas9-mediated disruption of PD-1 on human T cells for adoptive cellular therapies of EBV positive gastric cancer.
Su S, Zou Z, Chen F, Ding N, Du J, Shao J, Li L, Fu Y, Hu B, Yang Y, Sha H, Meng F, Wei J, Huang X, Liu B
Oncoimmunology 2017;6(1):e1249558
Oncoimmunology 2017;6(1):e1249558
Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy.
Feyaerts D, Benner M, van Cranenbroek B, van der Heijden OWH, Joosten I, van der Molen RG
Scientific reports 2017 Jun 6;7(1):2884
Scientific reports 2017 Jun 6;7(1):2884
Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans.
Rathod KS, Kapil V, Velmurugan S, Khambata RS, Siddique U, Khan S, Van Eijl S, Gee LC, Bansal J, Pitrola K, Shaw C, D'Acquisto F, Colas RA, Marelli-Berg F, Dalli J, Ahluwalia A
The Journal of clinical investigation 2017 Jan 3;127(1):169-182
The Journal of clinical investigation 2017 Jan 3;127(1):169-182
IL12-mediated sensitizing of T-cell receptor-dependent and -independent tumor cell killing.
Braun M, Ress ML, Yoo YE, Scholz CJ, Eyrich M, Schlegel PG, Wölfl M
Oncoimmunology 2016 Jul;5(7):e1188245
Oncoimmunology 2016 Jul;5(7):e1188245
CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients.
Su S, Hu B, Shao J, Shen B, Du J, Du Y, Zhou J, Yu L, Zhang L, Chen F, Sha H, Cheng L, Meng F, Zou Z, Huang X, Liu B
Scientific reports 2016 Jan 28;6:20070
Scientific reports 2016 Jan 28;6:20070
Common gamma chain cytokines promote rapid in vitro expansion of allo-specific human CD8+ suppressor T cells.
Yu Y, Zitzner JR, Houlihan J, Herrera N, Xu L, Miller J, Mathew JM, Tambur AR, Luo X
PloS one 2011;6(12):e28948
PloS one 2011;6(12):e28948
Human CD8+CXCR3+ T cells have the same function as murine CD8+CD122+ Treg.
Shi Z, Okuno Y, Rifa'i M, Endharti AT, Akane K, Isobe K, Suzuki H
European journal of immunology 2009 Aug;39(8):2106-19
European journal of immunology 2009 Aug;39(8):2106-19
Reciprocal CD4+ T-cell balance of effector CD62Llow CD4+ and CD62LhighCD25+ CD4+ regulatory T cells in small cell lung cancer reflects disease stage.
Koyama K, Kagamu H, Miura S, Hiura T, Miyabayashi T, Itoh R, Kuriyama H, Tanaka H, Tanaka J, Yoshizawa H, Nakata K, Gejyo F
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Nov 1;14(21):6770-9
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Nov 1;14(21):6770-9
L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts.
Jutila MA, Kurk S, Jackiw L, Knibbs RN, Stoolman LM
Journal of immunology (Baltimore, Md. : 1950) 2002 Aug 15;169(4):1768-73
Journal of immunology (Baltimore, Md. : 1950) 2002 Aug 15;169(4):1768-73
Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule.
Kishimoto TK, Jutila MA, Butcher EC
Proceedings of the National Academy of Sciences of the United States of America 1990 Mar;87(6):2244-8
Proceedings of the National Academy of Sciences of the United States of America 1990 Mar;87(6):2244-8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0049-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD62L (L-Selectin) FITC (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1. Activated CD8 + T cells in either normal or hypoxic culture. Naive CD8 + T cells derived from a healthy donor were cultured in human T-activator CD3/CD28 and IL-2 containing medium for 8 days. ( A ) in 20% O 2 condition and ( B ) in 1% O 2 condition. The 7-AAD-negative cells in the area gated as P1 in the FSC/SSC panel were considered as living cells. The expression pattern of CD45RA, CD62L, and CD127 of living cells were analyzed by FACS. The numbers on the FACS-plot panel mean the frequencies (%) of population of cells. We repeated this experiment four times.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Reduced inflammatory cell activation state in cantharidin-induced blister exudates in female compared with male healthy volunteers. Mean fluorescence intensity (MFI) of the expression molecules CD162, CD62L, and CD11b on ( A ) neutrophils, ( B ) inflammatory monocytes, and ( C ) CD4 + and CD8 + T cells in healthy male ( n = 16) and female ( n = 16) volunteers. Data are shown as mean +- SEM. Statistical significances determined using 2-way ANOVA, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001; followed by Sidak's post tests, # P < 0.05, ## P < 0.01, and #### P < 0.0001 comparing the sexes at each time point for all the panels.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Active NK cells with a unique transcriptome profile. a Top two enriched gene sets (ranked by normalized enrichment score) of five different datasets from GSEA of the ""Inflamed NK"" cluster compared to the rest of the cells were plotted. b The expression of CD69 in the BM sample was shown as a violin plot. The y -axis represents log-normalized expression value. c Module score was calculated using up-regulated DEGs of ""Active NK"" (left) or ""Inflamed NK"" (right) cluster from BM sample and plotted via violin plots. d Up-regulated IEGs from ""Active NK"" cluster were plotted using heatmap of the BM sample. e The expression of CXCR4 in the BM sample was shown as a violin plot. The y -axis represents log-normalized expression value. f Percentage of CXCR4 + NK cells (gated on Lin - CD56 + cells) was evaluated via flow cytometry. g The expression of CXCR4 in CD57 +/- , CD62L +/- , or NKG2A +/- CD56 dim NK populations from BM was assessed via flow cytometry (top). Percentage of CXCR4 + cells within each population were quantified (bottom). n >= 6 from two to five independent experiments. Paired Student's t test was used for the statistical analysis. * P < 0.05; ** p < 0.01; *** p < 0.001; n.s. stands for ""not significant."" Source data for f and g are provided as a Source Data file. See also Supplementary Fig. 5
- Conjugate
- Green dye