17-0629-42
antibody from Invitrogen Antibodies
Targeting: SELL
CD62L, hLHRc, LAM-1, LAM1, Leu-8, LNHR, LSEL, Lyam-1, LYAM1, PLNHR
Antibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [7]
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- Product number
- 17-0629-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.
- Antibody clone number
- DREG-56 (DREG56)
- Concentration
- 5 µL/Test
Submitted references Induction of memory-like CD8+ T cells and CD4+ T cells from human naive T cells in culture.
Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.
IL-17 inhibits CXCL9/10-mediated recruitment of CD8(+) cytotoxic T cells and regulatory T cells to colorectal tumors.
n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.
Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome.
Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans.
Type III Interferon-Mediated Signaling Is Critical for Controlling Live Attenuated Yellow Fever Virus Infection In Vivo.
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
Blocking MHC class II on human endothelium mitigates acute rejection.
Immune responses induced by T-cell vaccination in patients with rheumatoid arthritis.
The CD14+CD16+ inflammatory monocyte subset displays increased mitochondrial activity and effector function during acute Plasmodium vivax malaria.
Neutrophil paralysis in Plasmodium vivax malaria.
Differential expression of CD300a/c on human TH1 and TH17 cells.
Matrix metalloproteinase-2 conditions human dendritic cells to prime inflammatory T(H)2 cells via an IL-12- and OX40L-dependent pathway.
Monocyte surface expression of Fcgamma receptor RI (CD64), a biomarker reflecting type-I interferon levels in systemic lupus erythematosus.
Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription factor eomesodermin.
Increased expression of FcgammaRI/CD64 on circulating monocytes parallels ongoing inflammation and nephritis in lupus.
L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts.
Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule.
Tokumoto Y, Araki Y, Narizuka Y, Mizuno Y, Ohshima S, Mimura T
Clinical and experimental immunology 2022 Jan 28;207(1):95-103
Clinical and experimental immunology 2022 Jan 28;207(1):95-103
Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.
Yang C, Siebert JR, Burns R, Gerbec ZJ, Bonacci B, Rymaszewski A, Rau M, Riese MJ, Rao S, Carlson KS, Routes JM, Verbsky JW, Thakar MS, Malarkannan S
Nature communications 2019 Sep 2;10(1):3931
Nature communications 2019 Sep 2;10(1):3931
IL-17 inhibits CXCL9/10-mediated recruitment of CD8(+) cytotoxic T cells and regulatory T cells to colorectal tumors.
Chen J, Ye X, Pitmon E, Lu M, Wan J, Jellison ER, Adler AJ, Vella AT, Wang K
Journal for immunotherapy of cancer 2019 Nov 27;7(1):324
Journal for immunotherapy of cancer 2019 Nov 27;7(1):324
n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.
Wu B, Wang L, Jiang L, Dong L, Xu F, Lu Y, Jin J, Wang Z, Liang G, Shan X
Molecular medicine reports 2018 Jan;17(1):179-185
Molecular medicine reports 2018 Jan;17(1):179-185
Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome.
Douam F, Hrebikova G, Albrecht YE, Sellau J, Sharon Y, Ding Q, Ploss A
Nature communications 2017 Mar 14;8:14781
Nature communications 2017 Mar 14;8:14781
Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans.
Rathod KS, Kapil V, Velmurugan S, Khambata RS, Siddique U, Khan S, Van Eijl S, Gee LC, Bansal J, Pitrola K, Shaw C, D'Acquisto F, Colas RA, Marelli-Berg F, Dalli J, Ahluwalia A
The Journal of clinical investigation 2017 Jan 3;127(1):169-182
The Journal of clinical investigation 2017 Jan 3;127(1):169-182
Type III Interferon-Mediated Signaling Is Critical for Controlling Live Attenuated Yellow Fever Virus Infection In Vivo.
Douam F, Soto Albrecht YE, Hrebikova G, Sadimin E, Davidson C, Kotenko SV, Ploss A
mBio 2017 Aug 15;8(4)
mBio 2017 Aug 15;8(4)
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
Su S, Liao J, Liu J, Huang D, He C, Chen F, Yang L, Wu W, Chen J, Lin L, Zeng Y, Ouyang N, Cui X, Yao H, Su F, Huang JD, Lieberman J, Liu Q, Song E
Cell research 2017 Apr;27(4):461-482
Cell research 2017 Apr;27(4):461-482
Blocking MHC class II on human endothelium mitigates acute rejection.
Abrahimi P, Qin L, Chang WG, Bothwell AL, Tellides G, Saltzman WM, Pober JS
JCI insight 2016;1(1)
JCI insight 2016;1(1)
Immune responses induced by T-cell vaccination in patients with rheumatoid arthritis.
Ivanova I, Seledtsova G, Mamaev S, Shishkov A, Seledtsov V
Human vaccines & immunotherapeutics 2014;10(5):1221-7
Human vaccines & immunotherapeutics 2014;10(5):1221-7
The CD14+CD16+ inflammatory monocyte subset displays increased mitochondrial activity and effector function during acute Plasmodium vivax malaria.
Antonelli LR, Leoratti FM, Costa PA, Rocha BC, Diniz SQ, Tada MS, Pereira DB, Teixeira-Carvalho A, Golenbock DT, Gonçalves R, Gazzinelli RT
PLoS pathogens 2014 Sep;10(9):e1004393
PLoS pathogens 2014 Sep;10(9):e1004393
Neutrophil paralysis in Plasmodium vivax malaria.
Leoratti FM, Trevelin SC, Cunha FQ, Rocha BC, Costa PA, Gravina HD, Tada MS, Pereira DB, Golenbock DT, Antonelli LR, Gazzinelli RT
PLoS neglected tropical diseases 2012;6(6):e1710
PLoS neglected tropical diseases 2012;6(6):e1710
Differential expression of CD300a/c on human TH1 and TH17 cells.
Simhadri VR, Mariano JL, Zhou Q, DeBell KE, Borrego F
BMC immunology 2011 Nov 2;12:62
BMC immunology 2011 Nov 2;12:62
Matrix metalloproteinase-2 conditions human dendritic cells to prime inflammatory T(H)2 cells via an IL-12- and OX40L-dependent pathway.
Godefroy E, Manches O, Dréno B, Hochman T, Rolnitzky L, Labarrière N, Guilloux Y, Goldberg J, Jotereau F, Bhardwaj N
Cancer cell 2011 Mar 8;19(3):333-46
Cancer cell 2011 Mar 8;19(3):333-46
Monocyte surface expression of Fcgamma receptor RI (CD64), a biomarker reflecting type-I interferon levels in systemic lupus erythematosus.
Li Y, Lee PY, Kellner ES, Paulus M, Switanek J, Xu Y, Zhuang H, Sobel ES, Segal MS, Satoh M, Reeves WH
Arthritis research & therapy 2010;12(3):R90
Arthritis research & therapy 2010;12(3):R90
Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription factor eomesodermin.
Narayanan S, Silva R, Peruzzi G, Alvarez Y, Simhadri VR, Debell K, Coligan JE, Borrego F
PloS one 2010 May 13;5(5):e10636
PloS one 2010 May 13;5(5):e10636
Increased expression of FcgammaRI/CD64 on circulating monocytes parallels ongoing inflammation and nephritis in lupus.
Li Y, Lee PY, Sobel ES, Narain S, Satoh M, Segal MS, Reeves WH, Richards HB
Arthritis research & therapy 2009;11(1):R6
Arthritis research & therapy 2009;11(1):R6
L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts.
Jutila MA, Kurk S, Jackiw L, Knibbs RN, Stoolman LM
Journal of immunology (Baltimore, Md. : 1950) 2002 Aug 15;169(4):1768-73
Journal of immunology (Baltimore, Md. : 1950) 2002 Aug 15;169(4):1768-73
Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule.
Kishimoto TK, Jutila MA, Butcher EC
Proceedings of the National Academy of Sciences of the United States of America 1990 Mar;87(6):2244-8
Proceedings of the National Academy of Sciences of the United States of America 1990 Mar;87(6):2244-8
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Supportive validation
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- Staining of normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0048-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD62L (L-Selectin) APC (right). Cells in the lymphocyte gate were used for analysis.
Supportive validation
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- Fig. 1. Activated CD8 + T cells in either normal or hypoxic culture. Naive CD8 + T cells derived from a healthy donor were cultured in human T-activator CD3/CD28 and IL-2 containing medium for 8 days. ( A ) in 20% O 2 condition and ( B ) in 1% O 2 condition. The 7-AAD-negative cells in the area gated as P1 in the FSC/SSC panel were considered as living cells. The expression pattern of CD45RA, CD62L, and CD127 of living cells were analyzed by FACS. The numbers on the FACS-plot panel mean the frequencies (%) of population of cells. We repeated this experiment four times.
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- Figure 5 Reduced inflammatory cell activation state in cantharidin-induced blister exudates in female compared with male healthy volunteers. Mean fluorescence intensity (MFI) of the expression molecules CD162, CD62L, and CD11b on ( A ) neutrophils, ( B ) inflammatory monocytes, and ( C ) CD4 + and CD8 + T cells in healthy male ( n = 16) and female ( n = 16) volunteers. Data are shown as mean +- SEM. Statistical significances determined using 2-way ANOVA, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001; followed by Sidak's post tests, # P < 0.05, ## P < 0.01, and #### P < 0.0001 comparing the sexes at each time point for all the panels.
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- Fig. 4 Active NK cells with a unique transcriptome profile. a Top two enriched gene sets (ranked by normalized enrichment score) of five different datasets from GSEA of the ""Inflamed NK"" cluster compared to the rest of the cells were plotted. b The expression of CD69 in the BM sample was shown as a violin plot. The y -axis represents log-normalized expression value. c Module score was calculated using up-regulated DEGs of ""Active NK"" (left) or ""Inflamed NK"" (right) cluster from BM sample and plotted via violin plots. d Up-regulated IEGs from ""Active NK"" cluster were plotted using heatmap of the BM sample. e The expression of CXCR4 in the BM sample was shown as a violin plot. The y -axis represents log-normalized expression value. f Percentage of CXCR4 + NK cells (gated on Lin - CD56 + cells) was evaluated via flow cytometry. g The expression of CXCR4 in CD57 +/- , CD62L +/- , or NKG2A +/- CD56 dim NK populations from BM was assessed via flow cytometry (top). Percentage of CXCR4 + cells within each population were quantified (bottom). n >= 6 from two to five independent experiments. Paired Student's t test was used for the statistical analysis. * P < 0.05; ** p < 0.01; *** p < 0.001; n.s. stands for ""not significant."" Source data for f and g are provided as a Source Data file. See also Supplementary Fig. 5
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- Fig. 6 Blockade of IL-17 signaling in CRC results in enhanced immune checkpoint signaling. a q-RT-PCR analysis of normal colon tissue and colorectal tumors from Cdx2-Cre + /Apc F/+ mice that harbor heterozygous or alleles of Il17ra gene ( n = 12). b 4-month-old Cdx2-Cre + /Apc F/+ mice received i.p. injection of 100 mug isotype or anti-IL-17A antibodies every 3 days for 1 month. Mice were sacrificed for q-RT-PCR analysis ( n = 9). c Cdx2-Cre-ERT2 + /Apc F/F mice that were Il17ra +/- or Il17ra -/- were sacrificed 5 weeks after tamoxifen-induced Apc ablation, and their MLN and tumors were subjected to q-RT-PCR analysis ( n = 5 for MLN, 11 for tumor). d Cdx2-Cre-ERT2 + /Apc F/F mice that were Il17ra -/- or Il17ra +/- were sacrificed 5 weeks after tamoxifen-induced Apc ablation, and their MLN and tumors were subjected to flow cytometry analysis. n = 5. Data represent means +- S.E.M. * p < 0.05 in Students' t test