25-0629-41
antibody from Invitrogen Antibodies
Targeting: SELL
CD62L, hLHRc, LAM-1, LAM1, Leu-8, LNHR, LSEL, Lyam-1, LYAM1, PLNHR
Antibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [6]
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Validation data
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- Product number
- 25-0629-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), PE-Cyanine7, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues. Applications Reported: This DREG-56 (DREG56) antibody has been reported for use in flow cytometric analysis. Applications Tested: This DREG-56 (DREG56) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- DREG-56 (DREG56)
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Induction of memory-like CD8+ T cells and CD4+ T cells from human naive T cells in culture.
Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.
TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb.
Outcomes of controlled human malaria infection after BCG vaccination.
n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.
Functional T cells targeting tumor-associated antigens are predictive for recurrence-free survival of patients with radically operated non-small cell lung cancer.
Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans.
Modulation of Endoplasmic Reticulum Stress Controls CD4(+) T-cell Activation and Antitumor Function.
Immunodominant Dengue Virus-Specific CD8+ T Cell Responses Are Associated with a Memory PD-1+ Phenotype.
Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand.
Impact of malaria preexposure on antiparasite cellular and humoral immune responses after controlled human malaria infection.
PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer.
Efficient and reproducible generation of tumour-infiltrating lymphocytes for renal cell carcinoma.
Dendritic cell-MHC class II and Itk regulate functional development of regulatory innate memory CD4+ T cells in bone marrow transplantation.
Proportions of CD4+ memory T cells are altered in individuals chronically infected with Schistosoma haematobium.
Longevity and composition of cellular immune responses following experimental Plasmodium falciparum malaria infection in humans.
Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.
TRAIL deficiency does not rescue impaired CD8+ T cell memory generated in the absence of CD4+ T cell help.
Vaccines based on novel adeno-associated virus vectors elicit aberrant CD8+ T-cell responses in mice.
L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts.
Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule.
Tokumoto Y, Araki Y, Narizuka Y, Mizuno Y, Ohshima S, Mimura T
Clinical and experimental immunology 2022 Jan 28;207(1):95-103
Clinical and experimental immunology 2022 Jan 28;207(1):95-103
Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.
Yang C, Siebert JR, Burns R, Gerbec ZJ, Bonacci B, Rymaszewski A, Rau M, Riese MJ, Rao S, Carlson KS, Routes JM, Verbsky JW, Thakar MS, Malarkannan S
Nature communications 2019 Sep 2;10(1):3931
Nature communications 2019 Sep 2;10(1):3931
TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb.
Ma X, Yang T, Luo Y, Wu L, Jiang Y, Song Z, Pan T, Liu B, Liu G, Liu J, Yu F, He Z, Zhang W, Yang J, Liang L, Guan Y, Zhang X, Li L, Cai W, Tang X, Gao S, Deng K, Zhang H
eLife 2019 Jan 17;8
eLife 2019 Jan 17;8
Outcomes of controlled human malaria infection after BCG vaccination.
Walk J, de Bree LCJ, Graumans W, Stoter R, van Gemert GJ, van de Vegte-Bolmer M, Teelen K, Hermsen CC, Arts RJW, Behet MC, Keramati F, Moorlag SJCFM, Yang ASP, van Crevel R, Aaby P, de Mast Q, van der Ven AJAM, Stabell Benn C, Netea MG, Sauerwein RW
Nature communications 2019 Feb 20;10(1):874
Nature communications 2019 Feb 20;10(1):874
n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.
Wu B, Wang L, Jiang L, Dong L, Xu F, Lu Y, Jin J, Wang Z, Liang G, Shan X
Molecular medicine reports 2018 Jan;17(1):179-185
Molecular medicine reports 2018 Jan;17(1):179-185
Functional T cells targeting tumor-associated antigens are predictive for recurrence-free survival of patients with radically operated non-small cell lung cancer.
Safi S, Yamauchi Y, Rathinasamy A, Stamova S, Eichhorn M, Warth A, Rauch G, Dienemann H, Hoffmann H, Beckhove P
Oncoimmunology 2017;6(11):e1360458
Oncoimmunology 2017;6(11):e1360458
Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans.
Rathod KS, Kapil V, Velmurugan S, Khambata RS, Siddique U, Khan S, Van Eijl S, Gee LC, Bansal J, Pitrola K, Shaw C, D'Acquisto F, Colas RA, Marelli-Berg F, Dalli J, Ahluwalia A
The Journal of clinical investigation 2017 Jan 3;127(1):169-182
The Journal of clinical investigation 2017 Jan 3;127(1):169-182
Modulation of Endoplasmic Reticulum Stress Controls CD4(+) T-cell Activation and Antitumor Function.
Thaxton JE, Wallace C, Riesenberg B, Zhang Y, Paulos CM, Beeson CC, Liu B, Li Z
Cancer immunology research 2017 Aug;5(8):666-675
Cancer immunology research 2017 Aug;5(8):666-675
Immunodominant Dengue Virus-Specific CD8+ T Cell Responses Are Associated with a Memory PD-1+ Phenotype.
de Alwis R, Bangs DJ, Angelo MA, Cerpas C, Fernando A, Sidney J, Peters B, Gresh L, Balmaseda A, de Silva AD, Harris E, Sette A, Weiskopf D
Journal of virology 2016 May;90(9):4771-4779
Journal of virology 2016 May;90(9):4771-4779
Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand.
Tomić A, Varanasi PR, Golemac M, Malić S, Riese P, Borst EM, Mischak-Weissinger E, Guzmán CA, Krmpotić A, Jonjić S, Messerle M
PLoS pathogens 2016 Dec;12(12):e1006015
PLoS pathogens 2016 Dec;12(12):e1006015
Impact of malaria preexposure on antiparasite cellular and humoral immune responses after controlled human malaria infection.
Obiero JM, Shekalaghe S, Hermsen CC, Mpina M, Bijker EM, Roestenberg M, Teelen K, Billingsley PF, Sim BK, James ER, Daubenberger CA, Hoffman SL, Abdulla S, Sauerwein RW, Scholzen A
Infection and immunity 2015 May;83(5):2185-96
Infection and immunity 2015 May;83(5):2185-96
PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer.
Severson JJ, Serracino HS, Mateescu V, Raeburn CD, McIntyre RC Jr, Sams SB, Haugen BR, French JD
Cancer immunology research 2015 Jun;3(6):620-30
Cancer immunology research 2015 Jun;3(6):620-30
Efficient and reproducible generation of tumour-infiltrating lymphocytes for renal cell carcinoma.
Baldan V, Griffiths R, Hawkins RE, Gilham DE
British journal of cancer 2015 Apr 28;112(9):1510-8
British journal of cancer 2015 Apr 28;112(9):1510-8
Dendritic cell-MHC class II and Itk regulate functional development of regulatory innate memory CD4+ T cells in bone marrow transplantation.
Huang W, Qi Q, Hu J, Huang F, Laufer TM, August A
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3435-3441
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3435-3441
Proportions of CD4+ memory T cells are altered in individuals chronically infected with Schistosoma haematobium.
Nausch N, Bourke CD, Appleby LJ, Rujeni N, Lantz O, Trottein F, Midzi N, Mduluza T, Mutapi F
Scientific reports 2012;2:472
Scientific reports 2012;2:472
Longevity and composition of cellular immune responses following experimental Plasmodium falciparum malaria infection in humans.
Teirlinck AC, McCall MB, Roestenberg M, Scholzen A, Woestenenk R, de Mast Q, van der Ven AJ, Hermsen CC, Luty AJ, Sauerwein RW
PLoS pathogens 2011 Dec;7(12):e1002389
PLoS pathogens 2011 Dec;7(12):e1002389
Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.
Nagata S, Ise T, Pastan I
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7518-26
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7518-26
TRAIL deficiency does not rescue impaired CD8+ T cell memory generated in the absence of CD4+ T cell help.
Sacks JA, Bevan MJ
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):4570-6
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):4570-6
Vaccines based on novel adeno-associated virus vectors elicit aberrant CD8+ T-cell responses in mice.
Lin J, Zhi Y, Mays L, Wilson JM
Journal of virology 2007 Nov;81(21):11840-9
Journal of virology 2007 Nov;81(21):11840-9
L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts.
Jutila MA, Kurk S, Jackiw L, Knibbs RN, Stoolman LM
Journal of immunology (Baltimore, Md. : 1950) 2002 Aug 15;169(4):1768-73
Journal of immunology (Baltimore, Md. : 1950) 2002 Aug 15;169(4):1768-73
Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule.
Kishimoto TK, Jutila MA, Butcher EC
Proceedings of the National Academy of Sciences of the United States of America 1990 Mar;87(6):2244-8
Proceedings of the National Academy of Sciences of the United States of America 1990 Mar;87(6):2244-8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0048-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human CD62L (L-Seletin) PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
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- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
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- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
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- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1. Activated CD8 + T cells in either normal or hypoxic culture. Naive CD8 + T cells derived from a healthy donor were cultured in human T-activator CD3/CD28 and IL-2 containing medium for 8 days. ( A ) in 20% O 2 condition and ( B ) in 1% O 2 condition. The 7-AAD-negative cells in the area gated as P1 in the FSC/SSC panel were considered as living cells. The expression pattern of CD45RA, CD62L, and CD127 of living cells were analyzed by FACS. The numbers on the FACS-plot panel mean the frequencies (%) of population of cells. We repeated this experiment four times.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Reduced inflammatory cell activation state in cantharidin-induced blister exudates in female compared with male healthy volunteers. Mean fluorescence intensity (MFI) of the expression molecules CD162, CD62L, and CD11b on ( A ) neutrophils, ( B ) inflammatory monocytes, and ( C ) CD4 + and CD8 + T cells in healthy male ( n = 16) and female ( n = 16) volunteers. Data are shown as mean +- SEM. Statistical significances determined using 2-way ANOVA, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001; followed by Sidak's post tests, # P < 0.05, ## P < 0.01, and #### P < 0.0001 comparing the sexes at each time point for all the panels.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Active NK cells with a unique transcriptome profile. a Top two enriched gene sets (ranked by normalized enrichment score) of five different datasets from GSEA of the ""Inflamed NK"" cluster compared to the rest of the cells were plotted. b The expression of CD69 in the BM sample was shown as a violin plot. The y -axis represents log-normalized expression value. c Module score was calculated using up-regulated DEGs of ""Active NK"" (left) or ""Inflamed NK"" (right) cluster from BM sample and plotted via violin plots. d Up-regulated IEGs from ""Active NK"" cluster were plotted using heatmap of the BM sample. e The expression of CXCR4 in the BM sample was shown as a violin plot. The y -axis represents log-normalized expression value. f Percentage of CXCR4 + NK cells (gated on Lin - CD56 + cells) was evaluated via flow cytometry. g The expression of CXCR4 in CD57 +/- , CD62L +/- , or NKG2A +/- CD56 dim NK populations from BM was assessed via flow cytometry (top). Percentage of CXCR4 + cells within each population were quantified (bottom). n >= 6 from two to five independent experiments. Paired Student's t test was used for the statistical analysis. * P < 0.05; ** p < 0.01; *** p < 0.001; n.s. stands for ""not significant."" Source data for f and g are provided as a Source Data file. See also Supplementary Fig. 5