48-0629-42

antibody from Invitrogen Antibodies

Targeting: SELL CD62L, hLHRc, LAM-1, LAM1, Leu-8, LNHR, LSEL, Lyam-1, LYAM1, PLNHR

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

48-0629-42

Provider

Invitrogen Antibodies

Product name

CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), eFluor™ 450, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues. Applications Reported: This DREG-56 (DREG56) antibody has been reported for use in flow cytometric analysis. Applications Tested: This DREG-56 (DREG56) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

DREG-56 (DREG56)

Vial size

100 Tests

Concentration

5 µL/Test

Storage

4°C, store in dark, DO NOT FREEZE!

References

Submitted references

Induction of memory-like CD8+ T cells and CD4+ T cells from human naive T cells in culture.

Tokumoto Y, Araki Y, Narizuka Y, Mizuno Y, Ohshima S, Mimura T
Clinical and experimental immunology 2022 Jan 28;207(1):95-103

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Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.

Yang C, Siebert JR, Burns R, Gerbec ZJ, Bonacci B, Rymaszewski A, Rau M, Riese MJ, Rao S, Carlson KS, Routes JM, Verbsky JW, Thakar MS, Malarkannan S
Nature communications 2019 Sep 2;10(1):3931

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n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.

Wu B, Wang L, Jiang L, Dong L, Xu F, Lu Y, Jin J, Wang Z, Liang G, Shan X
Molecular medicine reports 2018 Jan;17(1):179-185

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Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans.

Rathod KS, Kapil V, Velmurugan S, Khambata RS, Siddique U, Khan S, Van Eijl S, Gee LC, Bansal J, Pitrola K, Shaw C, D'Acquisto F, Colas RA, Marelli-Berg F, Dalli J, Ahluwalia A
The Journal of clinical investigation 2017 Jan 3;127(1):169-182

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Tyrosine kinase 2 is not limiting human antiviral type III interferon responses.

Fuchs S, Kaiser-Labusch P, Bank J, Ammann S, Kolb-Kokocinski A, Edelbusch C, Omran H, Ehl S
European journal of immunology 2016 Nov;46(11):2639-2649

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L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts.

Jutila MA, Kurk S, Jackiw L, Knibbs RN, Stoolman LM
Journal of immunology (Baltimore, Md. : 1950) 2002 Aug 15;169(4):1768-73

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Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule.

Kishimoto TK, Jutila MA, Butcher EC
Proceedings of the National Academy of Sciences of the United States of America 1990 Mar;87(6):2244-8

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0049-42) and Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD62L (L-Selectin) eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0049-42) and Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD62L (L-Selectin) eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1. Activated CD8 + T cells in either normal or hypoxic culture. Naive CD8 + T cells derived from a healthy donor were cultured in human T-activator CD3/CD28 and IL-2 containing medium for 8 days. ( A ) in 20% O 2 condition and ( B ) in 1% O 2 condition. The 7-AAD-negative cells in the area gated as P1 in the FSC/SSC panel were considered as living cells. The expression pattern of CD45RA, CD62L, and CD127 of living cells were analyzed by FACS. The numbers on the FACS-plot panel mean the frequencies (%) of population of cells. We repeated this experiment four times.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 Reduced inflammatory cell activation state in cantharidin-induced blister exudates in female compared with male healthy volunteers. Mean fluorescence intensity (MFI) of the expression molecules CD162, CD62L, and CD11b on ( A ) neutrophils, ( B ) inflammatory monocytes, and ( C ) CD4 + and CD8 + T cells in healthy male ( n = 16) and female ( n = 16) volunteers. Data are shown as mean +- SEM. Statistical significances determined using 2-way ANOVA, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001; followed by Sidak's post tests, # P < 0.05, ## P < 0.01, and #### P < 0.0001 comparing the sexes at each time point for all the panels.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 Active NK cells with a unique transcriptome profile. a Top two enriched gene sets (ranked by normalized enrichment score) of five different datasets from GSEA of the ""Inflamed NK"" cluster compared to the rest of the cells were plotted. b The expression of CD69 in the BM sample was shown as a violin plot. The y -axis represents log-normalized expression value. c Module score was calculated using up-regulated DEGs of ""Active NK"" (left) or ""Inflamed NK"" (right) cluster from BM sample and plotted via violin plots. d Up-regulated IEGs from ""Active NK"" cluster were plotted using heatmap of the BM sample. e The expression of CXCR4 in the BM sample was shown as a violin plot. The y -axis represents log-normalized expression value. f Percentage of CXCR4 + NK cells (gated on Lin - CD56 + cells) was evaluated via flow cytometry. g The expression of CXCR4 in CD57 +/- , CD62L +/- , or NKG2A +/- CD56 dim NK populations from BM was assessed via flow cytometry (top). Percentage of CXCR4 + cells within each population were quantified (bottom). n >= 6 from two to five independent experiments. Paired Student's t test was used for the statistical analysis. * P < 0.05; ** p < 0.01; *** p < 0.001; n.s. stands for ""not significant."" Source data for f and g are provided as a Source Data file. See also Supplementary Fig. 5