PA5-95721
antibody from Invitrogen Antibodies
Targeting: SELL
CD62L, hLHRc, LAM-1, LAM1, Leu-8, LNHR, LSEL, Lyam-1, LYAM1, PLNHR
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-95721 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD62L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: MGCRRTREGP SKAMIFPWKC QSTQRDLWNI FKLWGWTMLC CDFLAHHGTD CWTYHYSEKP MNWQRARRFC RDNYTDLVAI QNKAEIEYLE KTLPFSRSYY; Positive Samples: Jurkat, Raji, Mouse spleen, Mouse thymus, Rat thymus; Cellular Location: Membrane, Single-pass type I membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.52 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of L-Selectin (CD62L) in extracts of various cell lines using CD62L Polyclonal Antibody (Product # PA5-95721) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of L-Selectin (CD62L) in paraffin-embedded rat testis using CD62L Polyclonal Antibody (Product # PA5-95721) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of L-Selectin (CD62L) in paraffin-embedded human tonsil using CD62L Polyclonal Antibody (Product # PA5-95721) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of L-Selectin (CD62L) in paraffin-embedded human gastric cancer using CD62L Polyclonal Antibody (Product # PA5-95721) at a dilution of 1:100.