NBP1-42795

antibody from Novus Biologicals

Targeting: SELL CD62L, hLHRc, LAM-1, LAM1, Leu-8, LNHR, LSEL, Lyam-1, LYAM1, PLNHR

Western blot

Immunoprecipitation

Immunohistochemistry

Antibody data

Submit

Validation data

Product number: NBP1-42795 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NBP1-42795, RRID:AB_10005932
Product name: Mouse Monoclonal L-Selectin/CD62L Antibody
Antibody type: Monoclonal
Description: Protein A purified. The mouse monoclonal antibody DREG56 recognizes CD62L/ L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells. HLDA V
Reactivity: Human
Host: Mouse
Isotype: IgG
Vial size: 0.1 mg
Concentration: 1 mg/ml
Storage: Store at 4C. Do not freeze.

SELL protein structure - NBP1-42795 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Analysis using the DyLight 405 conjugate of NBP1-42795. Staining of L-Selectin in human PBMCs using anti-L-Selectin antibody. Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Analysis using the PE/Cy5 conjugate of NBP1-42795. Staining of normal human peripheral blood cells with PE-Cy5 Mouse IgG1 kappa Isotype Control (open histogram) or Anti-Human CD62L (L-Selectin) PE-Cy5 (filled histogram). Cells in the lymphocyte gate were used for analysis.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Analysis using the FITC conjugate of NBP1-42795. Staining of normal human peripheral blood cells with Anti-Human CD4 APC and Mouse IgG1 K Isotype Control FITC (left) or Anti-Human CD62L (L-Selectin) FITC (right). Cells in the lymphocyte gate were used for analysis.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Analysis using the PE conjugate of NBP1-42795. Staining of L-Selectin in human PBMC using anti-L-Selectin antibody. The primary antibody was used at a dilution of 1:100, incubated for 25 minutes at 4C. Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Analysis using the PE conjugate of NBP1-42795. See Notes

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Analysis using the PE/Cy7 conjugate of NBP1-42795. Staining of normal human peripheral blood cells with Anti-Human CD4 FITC and Mouse IgG1 ? Isotype Control PE-Cy7(left) or Anti-Human CD62L (L-Seletin) PE-Cy7 (right).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Staining of CD62L in human peripheral blood with anti-CD62L (DREG56) FITC.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Surface staining of CD62L in human peripheral blood with anti-CD62L (DREG56) PerCP-CyTM5.5.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Surface staining of CD62L in human peripheral blood with anti-CD62L (DREG56) Alexa Fluor® 700.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.