Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- ARP32005_T100 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#ARP32005_T100, RRID:AB_841692
- Product name
- SMAD4 antibody - middle region (ARP32005_T100)
- Antibody type
- Polyclonal
- Antigen
- The immunogen is a synthetic peptide directed towards the middle region of human SMAD4
- Description
- Protein A purified
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Goat, Guinea Pig, Horse, Rabbit, Sheep
- Host
- Rabbit
- Vial size
- 100 μl
- Concentration
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Storage
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Sample Type: Human nuclear cell extracts (30ug) Primary Dilution: 1:1000 Secondary Antibody: anti-Rabbit HRP Secondary Dilution: 1:20000 Image Submitted By: Katarina Luciakova Cancer Research Institute See Customer Feedback tab for detailed information.
- Sample type
- U2OS nuclear extracts
- Primary Ab dilution
- 1:1000
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- SMAD4 antibody - middle region (ARP32005_T100) validated by WB using U2OS nuclear extracts
- Sample type
- U2OS nuclear extracts
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- WB Suggested Anti-SMAD4 Antibody Titration: 1.25ug/ml, ELISA Titer: 1:312500, Positive Control: HepG2 cell lysate
- Sample type
- U2OS nuclear extracts
- Primary Ab dilution
- 1.25 µg/mL
- Protocol
- Protocol