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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09377PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09377PU-N, RRID:AB_2035968
- Product name
- anti SMAD4
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to a region near the carboxy terminus of human SMAD4 protein
- Reactivity
- Human, Mouse, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.2 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using affinity purified anti-SMAD4 to detect over-expressed SMAD4 in transfected COS cells (lane 2). Lane 1 contains lysate from mock transfected COS cells. A doublet band is seen in the SMAD4 transfected lysate with the upper band (arrowhead) representing SMAD4 and the lower band being non-specific staining. The membrane was probed with tprimary antibody at a 1:1,000 dilution.
