AP09377PU-N

antibody from OriGene

Targeting: SMAD4 DPC4, MADH4

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

AP09377PU-N

Provider

OriGene

Product name

SMAD4 rabbit polyclonal antibody, Aff - Purified

Antibody type

Polyclonal

Description

SMAD4 rabbit polyclonal antibody, Aff - Purified

Host

Rabbit

Conjugate

Unconjugated

Epitope

SMAD4

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

1.2 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot using affinity purified anti-SMAD4 to detect over-expressed SMAD4 in transfected COS cells (lane 2). Lane 1 contains lysate from mock transfected COS cells. A doublet band is seen in the SMAD4 transfected lysate with the upper band (arrowhead) representing SMAD4 and the lower band being non-specific staining. The membrane was probed with tprimary antibody at a 1:1,000 dilution.

Validation comment

WB