Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AP09377PU-N - Provider product page
- Provider
- OriGene
- Product name
- SMAD4 rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- SMAD4 rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SMAD4
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.2 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-SMAD4 to detect over-expressed SMAD4 in transfected COS cells (lane 2). Lane 1 contains lysate from mock transfected COS cells. A doublet band is seen in the SMAD4 transfected lysate with the upper band (arrowhead) representing SMAD4 and the lower band being non-specific staining. The membrane was probed with tprimary antibody at a 1:1,000 dilution.
- Validation comment
- WB