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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- AP12711PU-N - Provider product page
- Provider
- OriGene
- Product name
- SMAD4 pThr277 rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- SMAD4 pThr277 rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SMAD4
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded Breast Carcinoma reacted with SMAD4 pThr277 (center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Dot blot analysis of anti-Phospho-SMAD4 pThr277 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
- Validation comment
- DB
