H00004089-M09

antibody from Abnova Corporation

Targeting: SMAD4 DPC4, MADH4

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004089-M09

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004089-M09, RRID:AB_581771

Product name

SMAD4 monoclonal antibody (M09), clone 3D7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant SMAD4.

Antigen sequence

SLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGF
PHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDS
VCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKD
EYVHD


Isotype

IgG

Antibody clone number

3D7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SMAD4 is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between AKT1 and SMAD4. Huh7 cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-SMAD4 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)