PAB11265

antibody from Abnova Corporation

Targeting: SMAD4 DPC4, MADH4

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB11265

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB11265, RRID:AB_1709910

Product name

SMAD4 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of SMAD4.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Smad4 is critical for self-renewal of hematopoietic stem cells.

Karlsson G, Blank U, Moody JL, Ehinger M, Singbrant S, Deng CX, Karlsson S
The Journal of experimental medicine 2007 Mar 19;204(3):467-74

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Inactivation of SMAD4 tumor suppressor gene during gastric carcinoma progression.

Wang LH, Kim SH, Lee JH, Choi YL, Kim YC, Park TS, Hong YC, Wu CF, Shin YK
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Jan 1;13(1):102-10

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Smad4 cooperates with lymphoid enhancer-binding factor 1/T cell-specific factor to increase c-myc expression in the absence of TGF-beta signaling.

Lim SK, Hoffmann FM
Proceedings of the National Academy of Sciences of the United States of America 2006 Dec 5;103(49):18580-5

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using SMAD4 polyclonal antibody (Cat # PAB11265) to detect over-expressed SMAD4 in transfected COS cells (lane 2).Lane 1 contains lysate from mock transfected COS cells.A doublet band is seen in the SMAD4 transfected lysate with the upper band (arrowhead) representing SMAD4 and the lower band being non-specific staining.The primary antibody at a 1:1,000 dilution.Personal Communication, Kathleen Flanders, CCR-NCI, Bethesda, MD.