NBP2-67372

antibody from Novus Biologicals

Targeting: SMAD4 DPC4, MADH4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-67372

Provider

Novus Biologicals

Product name

Rabbit Monoclonal Smad4 Antibody

Antibody type

Monoclonal

Description

Protein A purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Smad4 Antibody (SP05-05) [NBP2-67372] - Analysis of Smad4 on PC-12 cells lysates using anti-Smad4 antibody at 1/1,000 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Smad4 Antibody (SP05-05) [NBP2-67372] - Analysis of paraffin-embedded mouse brain tissue using anti-Smad4 antibody. Counter stained with hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Smad4 Antibody (SP05-05) [NBP2-67372] - Analysis of paraffin-embedded human lung cancer tissue using anti-Smad4 antibody. Counter stained with hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Smad4 Antibody (SP05-05) [NBP2-67372] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Smad4 antibody. Counter stained with hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Smad4 Antibody (SP05-05) [NBP2-67372] - Analysis of paraffin-embedded mouse colon tissue using anti-Smad4 antibody. Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Smad4 Antibody (SP05-05) [NBP2-67372] - Analysis of SH-SY-5Y cells with Smad4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.