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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- MA5-14300 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMAD4 Monoclonal Antibody (SMD46 (DCS-46))
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-14300 targets SMAD4 in IF, IP, and WB applications and shows reactivity with Human samples.
- Antibody clone number
- SMD46 (DCS-46)
- Concentration
- 0.2 mg/mL
Submitted references Pyruvate Dehydrogenase Kinase 4 Deficiency Results in Expedited Cellular Proliferation through E2F1-Mediated Increase of Cyclins.
Effect of DPC4 gene on invasion and metastasis of colorectal carcinoma cells.
Effect of DPC4 gene on invasion and metastasis of colorectal carcinoma cells.
Notch signaling modulates the nuclear localization of carboxy-terminal-phosphorylated smad2 and controls the competence of ectodermal cells for activin A.
Choiniere J, Wu J, Wang L
Molecular pharmacology 2017 Mar;91(3):189-196
Molecular pharmacology 2017 Mar;91(3):189-196
Effect of DPC4 gene on invasion and metastasis of colorectal carcinoma cells.
Xiao DS, Wen JF, Li JH, Wang KS, Hu ZL, Zhou JH, Deng ZH, Liu Y
Acta biochimica et biophysica Sinica 2006 Dec;38(12):883-92
Acta biochimica et biophysica Sinica 2006 Dec;38(12):883-92
Effect of DPC4 gene on invasion and metastasis of colorectal carcinoma cells.
Xiao DS, Wen JF, Li JH, Wang KS, Hu ZL, Zhou JH, Deng ZH, Liu Y
Acta biochimica et biophysica Sinica 2006 Dec;38(12):883-92
Acta biochimica et biophysica Sinica 2006 Dec;38(12):883-92
Notch signaling modulates the nuclear localization of carboxy-terminal-phosphorylated smad2 and controls the competence of ectodermal cells for activin A.
Abe T, Furue M, Kondow A, Matsuzaki K, Asashima M
Mechanisms of development 2005 May;122(5):671-80
Mechanisms of development 2005 May;122(5):671-80
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMAD4 was performed with 10 µg of HeLa cells transfected with Transfection Reagent alone (Lane 1), 100nM Non-Targeting control siRNA (Lane 2), or 100nM siRNA against SMAD4 (Lane 3). Proteins were resolved using a NuPAGE® Novex 4-12% Bis-Tris Gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and a protein size ladder. Proteins were wet transferred to a Pierce Nitrocellulose Membrane (Product # 88025) OR Pierce PVDF Membrane (Product # 88518) and blocked with Pierce Starting Block T20 (PBS) Blocking Buffer (Product # 37539) for 1 hour at room temperature. SMAD4 was detected at ~ 60 kDa using SMAD4 Mouse monoclonal antibody (Product # MA5-14300) diluted in Pierce Starting Block T20 (PBS) Blocking Buffer 4°C overnight on a rocking platform. Pierce Goat Anti-Mouse (Product # 31437) HRP-Conjugated Antibodies at a 1:2500 dilution were used and chemiluminescent detection was performed using Pierce Supersignal West Dura Maximum Sensitivity Substrate (Product # 37071). Relative density of the bands normalized to Actin (45 kDa). SMAD4 Antibody (Product # MA5-14300) confirms silencing of SMAD4 expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of SMAD4 using SMAD4 Monoclonal Antibody (Product # MA5-14300) on LS174T Cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of SMAD4 using SMAD4 Monoclonal Antibody (Product # MA5-14300) on Native Human LS174T Cells.
