Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-12695 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-SMAD4 (Thr277) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references YAP and TAZ are distinct effectors of corneal myofibroblast transformation.
TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.
Muppala S, Raghunathan VK, Jalilian I, Thomasy S, Murphy CJ
Experimental eye research 2019 Mar;180:102-109
Experimental eye research 2019 Mar;180:102-109
TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.
Huang SC, Wei PC, Hwang-Verslues WW, Kuo WH, Jeng YM, Hu CM, Shew JY, Huang CS, Chang KJ, Lee EY, Lee WH
EMBO molecular medicine 2017 Dec;9(12):1660-1680
EMBO molecular medicine 2017 Dec;9(12):1660-1680
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with TGF-beta (100 ng/mL, 30 min), using Phospho-SMAD4 (Thr277) Polyclonal Antibody (Product # PA5-12695) or Beta-actin.