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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB20971 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Smad4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects endogenous human Smad4 in Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human Smad1, 5, 6, 7, or 9.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q13485- Isotype
- IgG
- Antibody clone number
- 253327
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Smad4 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Human Smad4 Monoclonal Antibody (Catalog # MAB20971) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Smad4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
