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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
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- Product number
- NB100-63392 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-63392, RRID:AB_959638
- Product name
- Mouse Monoclonal Aminopeptidase N/CD13 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. NB100-63392 recognizes bovine CD13, a 150 kD type II membrane protein shown to be a metallopeptidase in humans. In cattle the antigen recognised by clone CC81 is primarily expressed on enterocytes and cells with a dendritic morphology in the small intestine. Clone CC81 also defines a subpopulation of dendritic cells in afferent lymph that are CC81 Ag +ve and SIRPalpha -ve, which show differences in their capacities to stimulate T cells and cytokine synthesis compared to the CC81 Ag -ve SIRPalpha +ve dendritic cells.
- Reactivity
- Bovine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.125 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Aminopeptidase N/CD13 Antibody (CC81) [NB100-63392] - Figure A. Purified mouse anti bovine CD13 detected with goat anti mouse IgG1 RPE and FITC conjugated mouse IgG2b isotype control. Figure B. Purified mouse anti bovine CD13 detected with goat anti mouse IgG1 RPE and FITC conjugated mouse anti bovine CD11b. All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Aminopeptidase N/CD13 Antibody (CC81) [NB100-63392] - Figure A. FITC conjugated mouse anti bovine CD11b and purified mouse IgG1 isotype control detected with RPE conjugated goat anti mouse IgG1. Figure B. FITC conjugated mouse anti bovine CD11b and purified CD13 detected with RPE conjugated goat anti mouse IgG1. All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
