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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- ABIN2616447 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Alanyl (Membrane) Aminopeptidase (ANPEP) antibody
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 22A5
- Vial size
- 100 μg
- Storage
- Store at 2°C to 8°C.
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Supportive validation
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- antibodies-online (provider)
- Main image
- Experimental details
- DNMT1 monomethyl Lys142 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (40 μg) probed with DNMT1 monomethyl Lys142 antibody (1:500).
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- antibodies-online (provider)
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- Experimental details
- DNMT1 monomethyl Lys142 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of DNMT1 monomethyl Lys142. Peptides corresponding to the immunogen and the unmodified version of the immunogen were spotted onto PVDF and probed with the antibody at 1:100,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Monomethyl Lys142 peptide. Lane 2: Dimethyl lysine 142 peptide. Lane 3: Trimethyl lysine142 peptide. Lane 4: Unmodified lysine 142 peptide.
