Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 14-0138-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD13 Monoclonal Antibody (WM-15 (WM15)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The WM-15 monoclonal antibody reacts with human CD13, also known as aminopeptidase N. CD13 is a transmembrane ectoenzyme occurring on monocytes and granulocytes but not lymphocytes in blood. It is also reportedly on a wide variety of cells and in a wide variety of tissues, including endothelial cells, epithelial cells, fibroblasts, kidney proximal tubules, intestine, and placenta. CD13 is reported to have many different functions including degradation of enkephalins and endorphins, terminal degradation of peptides, amino acid scavenging, antigen processing, and adhesion and migration of cells. Applications Reported: The WM-15 (WM15) antibody) has been reported for use in flow cytometric analysis, and microscopy. Applications Tested: The WM-15 (WM15) antibody has been tested by flow cytometric analysis of lysed whole blood. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- WM-15 (WM15)
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells.
ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells.
Induction of aminopeptidase N/CD13 on human lymphocytes after adhesion to fibroblast-like synoviocytes, endothelial cells, epithelial cells, and monocytes/macrophages.
Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N.
Zhu P, Wang Y, Wu J, Huang G, Liu B, Ye B, Du Y, Gao G, Tian Y, He L, Fan Z
Nature communications 2016 Dec 1;7:13608
Nature communications 2016 Dec 1;7:13608
ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells.
Zhu P, Wang Y, He L, Huang G, Du Y, Zhang G, Yan X, Xia P, Ye B, Wang S, Hao L, Wu J, Fan Z
The Journal of clinical investigation 2015 Oct 1;125(10):3795-808
The Journal of clinical investigation 2015 Oct 1;125(10):3795-808
Induction of aminopeptidase N/CD13 on human lymphocytes after adhesion to fibroblast-like synoviocytes, endothelial cells, epithelial cells, and monocytes/macrophages.
Riemann D, Kehlen A, Thiele K, Löhn M, Langner J
Journal of immunology (Baltimore, Md. : 1950) 1997 Apr 1;158(7):3425-32
Journal of immunology (Baltimore, Md. : 1950) 1997 Apr 1;158(7):3425-32
Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N.
Look AT, Ashmun RA, Shapiro LH, Peiper SC
The Journal of clinical investigation 1989 Apr;83(4):1299-307
The Journal of clinical investigation 1989 Apr;83(4):1299-307
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.5 µg of Mouse IgG1 Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-Human CD13 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the monocyte (left) and granulocyte (right) gates were used for analysis.