AP13740b

antibody from Abgent

Targeting: CD27 S152, TNFRSF7, Tp55

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP13740b

Provider

Abgent

Proper citation

Abgent Cat#AP13740b, RRID:AB_10893841

Product name

CD27 Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.49 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

CD27 Antibody (C-term) (Cat. #AP13740b) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the CD27 antibody detected the CD27 protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of CD27 (arrow) using rabbit polyclonal CD27 Antibody (C-term) (Cat. #AP13740b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CD27 gene.

Primary Ab dilution

1:1000

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

CD27 Antibody (C-term) (Cat. #AP13740b) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50