Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 124219 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#124219, RRID:AB_2565539
- Product name
- Alexa Fluor® 647 anti-mouse/rat/human CD27
- Antibody type
- Monoclonal
- Antigen
- Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA
- Description
- CD27 is also known as S152 and T14. A member of the tumor necrosis factor receptor (TNFR) superfamily, it is a 45 kD protein expressed on peripheral T cells, memory B cells, NK cells, and thymocyte subset. Through its ligand, CD70, CD27 plays a key role in T cell and B cell interactions. Additionally, ligation of CD27 on naïve T cells may be important in their maturation to effector cells.
- Reactivity
- Human, Mouse, Rat
- Conjugate
- Red dye
- Antibody clone number
- LG.3A10
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- C57Bl/6 mouse splenocytes were stained with CD27 (clone LG.3A10) Alexa Fluor® 647 (filled histogram) or Armenian Hamster IgG Alexa Fluor® 647 isotype control (open histogram).
- Conjugate
- Red dye