Q10065

antibody from Invitrogen Antibodies

Targeting: CD27 S152, TNFRSF7, Tp55

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

Q10065

Provider

Invitrogen Antibodies

Product name

CD27 Monoclonal Antibody (CLB-27/1), Qdot™ 605

Antibody type

Monoclonal

Antigen

Other

Description

Qdot™ Antibody (Ab) conjugates possess a bright fluorescence emission that makes them well suited for the detection of low-abundance extracellular proteins. Approximately the same size as R-phycoerythrin (R-PE) and compatible with existing organic fluorophore conjugates, Qdot™ Ab conjugates can be excited with any wavelength below their emission maximum, but are best excited by UV or violet light. The narrow, symmetric emission profiles of Qdot Ab conjugates allow for minimal compensation when using a single excitation source, and the very long stoke shifts enable better, more efficient multicolor assays using the 405 nm violet laser. Available in multiple colors for use in flow cytometry, these advantages make Qdot Ab conjugates powerful tools for antibody labeling and staining. Staining: Stain cells in any standard staining buffer, such as phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA). Use 1 L of Qdot antibody conjugate per 1 × 10^6 cells in a 100 µL staining volume (20 nM final concentration of Qdot™ nanocrystals). Qdot™ nanocrystal conjugates may be mixed with other antibodies, but use the diluted conjugates on the day of dilution. Qdot nanocrystal conjugates can be used for surface staining applications with most conventional sample preparation reagents, such as Cal-Lyse™ and FIX & PERM™ reagents, with minimal affect on fluorescence. We have observed that some batches of BD FACS™ Lysing Solution have been found to interfere with Qdot™ nanocrystal fluorescence. Each lot has been tested by flow cytometry using human peripheral blood leukocytes. This testing was performed using 1 L of antibody per 1 × 106 cells in a 100 L staining volume (20 nM final concentration of Qdot nanocrystals). Qdot nanocrystal concentration is assigned based on optical density. The isotype control for this antibody is mouse IgG2a, Cat. No. Q10014.

Antibody clone number

CLB-27/1

References

Submitted references

CD57(+) Memory T Cells Proliferate In Vivo.

Ahmed R, Miners KL, Lahoz-Beneytez J, Jones RE, Roger L, Baboonian C, Zhang Y, Wang ECY, Hellerstein MK, McCune JM, Baird DM, Price DA, Macallan DC, Asquith B, Ladell K
Cell reports 2020 Dec 15;33(11):108501

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Selective Loss of Early Differentiated, Highly Functional PD1high CD4 T Cells with HIV Progression.

Paris RM, Petrovas C, Ferrando-Martinez S, Moysi E, Boswell KL, Archer E, Yamamoto T, Ambrozak D, Casazza JP, Haubrich R, Connors M, Ake J, Kim JH, Koup RA
PloS one 2015;10(12):e0144767

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Longitudinal studies of a B cell-derived signature of tolerance in renal transplant recipients.

Newell KA, Asare A, Sanz I, Wei C, Rosenberg A, Gao Z, Kanaparthi S, Asare S, Lim N, Stahly M, Howell M, Knechtle S, Kirk A, Marks WH, Kawai T, Spitzer T, Tolkoff-Rubin N, Sykes M, Sachs DH, Cosimi AB, Burlingham WJ, Phippard D, Turka LA
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2015 Nov;15(11):2908-20

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Analysis of human B-cell responses following ChAd63-MVA MSP1 and AMA1 immunization and controlled malaria infection.

Elias SC, Choudhary P, de Cassan SC, Biswas S, Collins KA, Halstead FD, Bliss CM, Ewer KJ, Hodgson SH, Duncan CJ, Hill AV, Draper SJ
Immunology 2014 Apr;141(4):628-44

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Multiparameter flow cytometry and bioanalytics for B cell profiling in systemic lupus erythematosus.

Kaminski DA, Wei C, Rosenberg AF, Lee FE, Sanz I
Methods in molecular biology (Clifton, N.J.) 2012;900:109-34

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Decreased influenza-specific B cell responses in rheumatoid arthritis patients treated with anti-tumor necrosis factor.

Kobie JJ, Zheng B, Bryk P, Barnes M, Ritchlin CT, Tabechian DA, Anandarajah AP, Looney RJ, Thiele RG, Anolik JH, Coca A, Wei C, Rosenberg AF, Feng C, Treanor JJ, Lee FE, Sanz I
Arthritis research & therapy 2011;13(6):R209

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Qdot® 605 anti-human CD27 conjugate 605/20 band pass filter

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 CD57 - and CD57 + Memory T Cells Exhibit Similar Rates of Deuterium Incorporation (A) Schematic representation of the 2 H 2 O labeling protocol and sampling time points. (B) Experimental labeling data for CD57 - and CD57 + memory CD8 + T cells sampled from the HIV-1-infected volunteers in cohort 1. The corresponding flow cytometric gating strategy is shown in Figure S1 . (C) Successive panels depict the flow cytometric gating strategy used to sort CD57 - and CD57 + memory T cells from the CD4 + and CD8 + lineages (cohort 2). Lymphocytes were identified in a forward scatter-area versus side scatter-area plot, and single cells were identified in a forward scatter-area versus forward scatter-height plot. Boolean gates were drawn for analysis only to exclude fluorochrome aggregates. Viable CD3 + CD14 - CD19 - cells were then identified in the CD4 + and CD8 + lineages, and sort gates were fixed on CD57 - and CD57 + memory cells after exclusion of potentially naive CD27 bright CD45RO - cells. (D) Experimental labeling data for CD57 - and CD57 + memory CD4 + T cells sampled from the healthy volunteers in cohort 2. (E) Experimental labeling data for CD57 - and CD57 + memory CD8 + T cells sampled from the healthy volunteers in cohort 2.