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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB574 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CD27/TNFRSF7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CD27 in direct ELISAs and Western blots. In Western blots, does not cross-react with recombinant human (rh) CD27, rhCD30, rhCD40, rhDR3, rhDR6, rhFas Ligand, rhTRAIL R3, rhTRAIL R4, rhLT beta R, rhNGF-R1, rh4-1BB, rhGITR, rhTNFR1, rhTNF RII, rhBAFF, rhTAJ, rhEDA, rhOPG, rhRELT, rhRANK, recombinant mouse (rm) CD30, rmCD40, rmGITR, rmHVEM, rmRANK, rmFas, rmTRAIL R2, rm4-1BB, rmLT beta R, rmOPG, rmNGFR, rmDR3, rmBAFF R, rmEDAR, rmOX-40, rmTWEAK R, rmTNF R1, recombinant rat Fas, or recombinant feline Fas.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P41272- Isotype
- IgG
- Antibody clone number
- 137910
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse CD27/TNFRSF7 by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF Membrane was probed with 2 µg/mL of Mouse CD27/TNFRSF7 Monoclonal Antibody (Catalog # MAB574) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD27/TNFRSF7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD27/TNFRSF7 in Mouse Splenocytes. CD27/TNFRSF7 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD27/TNFRSF7 Monoclonal Antibody (Catalog # MAB574) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
