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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- F43213 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD27 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 196-225 from the human protein was used as the immunogen for this CD27 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the CD27 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- CD27 antibody western blot analysis in Ramos lysate. Predicted molecular weight ~29 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of CD27 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the CD27 gene.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- CD27 antibody flow cytometric analysis of Ramos cells (right histogram) compared to a negative control (left histogram). FITC-conjugated donkey-anti-rabbit secondary Ab was used for the analysis.
