H00000939-M01

antibody from Abnova Corporation

Targeting: CD27 S152, TNFRSF7, Tp55

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000939-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000939-M01, RRID:AB_425348

Product name

TNFRSF7 monoclonal antibody (M01), clone 1E2-A3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant TNFRSF7.

Antigen sequence

MARPHPWWLCVLGTLVGLSATPAPKSCPERHYWAQ
GKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVS
FSPDHHTRPHCESCRHCNSGLLVRNCTITANAECA
CRNGWQCRDKECTECDPLPNPSLTARSSQALSPHP
QPTHLPYVSEMLEARTAGHMQTLADFRQLPARTLS
THWPPQRSLCSSDFIRILVIFSGMFLVFTLAGALF
LHQRRKYRSNKGESPVEPAEPCRYSCPREEEGSTI
PIQEDYRKPEPACSP


Isotype

IgG

Antibody clone number

1E2-A3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

TNFRSF7 monoclonal antibody (M01), clone 1E2-A3 Western Blot analysis of TNFRSF7 expression in Jurkat ( Cat # L017V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of TNFRSF7 expression in transfected 293T cell line by TNFRSF7 monoclonal antibody (M01), clone 1E2-A3.Lane 1: TNFRSF7 transfected lysate(29.2 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CD27 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol