Antibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-0279-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD27 Monoclonal Antibody (O323), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction. Applications Reported: The O323 antibody has been reported for use in flow cytometric analysis. Applications Tested: This O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- O323
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Perturbed CD8(+) T cell immunity across universal influenza epitopes in the elderly.
Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.
Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals.
Maintenance of the EBV-specific CD8+ TCRαβ repertoire in immunosuppressed lung transplant recipients.
Antibody Tumor Targeting Is Enhanced by CD27 Agonists through Myeloid Recruitment.
Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.
Inclusion of Strep-tag II in design of antigen receptors for T-cell immunotherapy.
Detection of impaired IgG antibody formation facilitates the decision on early immunoglobulin replacement in hypogammaglobulinemic patients.
Surface Plasmon Resonance Analysis Shows an IgG-Isotype-Specific Defect in ABO Blood Group Antibody Formation in Patients with Common Variable Immunodeficiency.
Effect of Leflunomide on the Abnormal Expression of Lipid Rafts and F-Actin in B Lymphocytes from Patients with Systemic Lupus Erythematosus.
Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.
Comprehensive analysis of dengue virus-specific responses supports an HLA-linked protective role for CD8+ T cells.
Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.
Reciprocal polarization of T and B cells at the immunological synapse.
Alterations on peripheral blood B-cell subpopulations in very early arthritis patients.
Agricultural pesticide exposure and the molecular connection to lymphomagenesis.
Nguyen THO, Sant S, Bird NL, Grant EJ, Clemens EB, Koutsakos M, Valkenburg SA, Gras S, Lappas M, Jaworowski A, Crowe J, Loh L, Kedzierska K
Journal of leukocyte biology 2018 Feb;103(2):321-339
Journal of leukocyte biology 2018 Feb;103(2):321-339
Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.
Van Caeneghem Y, De Munter S, Tieppo P, Goetgeluk G, Weening K, Verstichel G, Bonte S, Taghon T, Leclercq G, Kerre T, Debets R, Vermijlen D, Abken H, Vandekerckhove B
Oncoimmunology 2017;6(3):e1283460
Oncoimmunology 2017;6(3):e1283460
Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals.
Magri G, Comerma L, Pybus M, Sintes J, Lligé D, Segura-Garzón D, Bascones S, Yeste A, Grasset EK, Gutzeit C, Uzzan M, Ramanujam M, van Zelm MC, Albero-González R, Vazquez I, Iglesias M, Serrano S, Márquez L, Mercade E, Mehandru S, Cerutti A
Immunity 2017 Jul 18;47(1):118-134.e8
Immunity 2017 Jul 18;47(1):118-134.e8
Maintenance of the EBV-specific CD8+ TCRαβ repertoire in immunosuppressed lung transplant recipients.
Nguyen TH, Bird NL, Grant EJ, Miles JJ, Thomas PG, Kotsimbos TC, Mifsud NA, Kedzierska K
Immunology and cell biology 2017 Jan;95(1):77-86
Immunology and cell biology 2017 Jan;95(1):77-86
Antibody Tumor Targeting Is Enhanced by CD27 Agonists through Myeloid Recruitment.
Turaj AH, Hussain K, Cox KL, Rose-Zerilli MJJ, Testa J, Dahal LN, Chan HTC, James S, Field VL, Carter MJ, Kim HJ, West JJ, Thomas LJ, He LZ, Keler T, Johnson PWM, Al-Shamkhani A, Thirdborough SM, Beers SA, Cragg MS, Glennie MJ, Lim SH
Cancer cell 2017 Dec 11;32(6):777-791.e6
Cancer cell 2017 Dec 11;32(6):777-791.e6
Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.
Kong F, Feng B, Zhang H, Rao H, Wang J, Cong X, Wei L
Experimental and therapeutic medicine 2017 Aug;14(2):1846-1852
Experimental and therapeutic medicine 2017 Aug;14(2):1846-1852
Inclusion of Strep-tag II in design of antigen receptors for T-cell immunotherapy.
Liu L, Sommermeyer D, Cabanov A, Kosasih P, Hill T, Riddell SR
Nature biotechnology 2016 Apr;34(4):430-4
Nature biotechnology 2016 Apr;34(4):430-4
Detection of impaired IgG antibody formation facilitates the decision on early immunoglobulin replacement in hypogammaglobulinemic patients.
Wolf HM, Thon V, Litzman J, Eibl MM
Frontiers in immunology 2015;6:32
Frontiers in immunology 2015;6:32
Surface Plasmon Resonance Analysis Shows an IgG-Isotype-Specific Defect in ABO Blood Group Antibody Formation in Patients with Common Variable Immunodeficiency.
Fischer MB, Wolfram W, Binder CJ, Böhmig GA, Wahrmann M, Eibl MM, Wolf HM
Frontiers in immunology 2015;6:211
Frontiers in immunology 2015;6:211
Effect of Leflunomide on the Abnormal Expression of Lipid Rafts and F-Actin in B Lymphocytes from Patients with Systemic Lupus Erythematosus.
Dong GF, Zhang X, He DN, Li L, Zhang GF
Journal of immunology research 2015;2015:832916
Journal of immunology research 2015;2015:832916
Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.
Ndungu FM, Lundblom K, Rono J, Illingworth J, Eriksson S, Färnert A
European journal of immunology 2013 Nov;43(11):2919-29
European journal of immunology 2013 Nov;43(11):2919-29
Comprehensive analysis of dengue virus-specific responses supports an HLA-linked protective role for CD8+ T cells.
Weiskopf D, Angelo MA, de Azeredo EL, Sidney J, Greenbaum JA, Fernando AN, Broadwater A, Kolla RV, De Silva AD, de Silva AM, Mattia KA, Doranz BJ, Grey HM, Shresta S, Peters B, Sette A
Proceedings of the National Academy of Sciences of the United States of America 2013 May 28;110(22):E2046-53
Proceedings of the National Academy of Sciences of the United States of America 2013 May 28;110(22):E2046-53
Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.
Illingworth J, Butler NS, Roetynck S, Mwacharo J, Pierce SK, Bejon P, Crompton PD, Marsh K, Ndungu FM
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
Reciprocal polarization of T and B cells at the immunological synapse.
Duchez S, Rodrigues M, Bertrand F, Valitutti S
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 1;187(9):4571-80
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 1;187(9):4571-80
Alterations on peripheral blood B-cell subpopulations in very early arthritis patients.
Moura RA, Weinmann P, Pereira PA, Caetano-Lopes J, Canhão H, Sousa E, Mourão AF, Rodrigues AM, Queiroz MV, Souto-Carneiro MM, Graça L, Fonseca JE
Rheumatology (Oxford, England) 2010 Jun;49(6):1082-92
Rheumatology (Oxford, England) 2010 Jun;49(6):1082-92
Agricultural pesticide exposure and the molecular connection to lymphomagenesis.
Agopian J, Navarro JM, Gac AC, Lecluse Y, Briand M, Grenot P, Gauduchon P, Ruminy P, Lebailly P, Nadel B, Roulland S
The Journal of experimental medicine 2009 Jul 6;206(7):1473-83
The Journal of experimental medicine 2009 Jul 6;206(7):1473-83
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD27 PE (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye