- 13-0279-82 - Invitrogen Antibodies CD27 antibody

Submitted references Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Wang Q, Sun Q, Chen Q, Li H, Liu D
Experimental and therapeutic medicine 2020 Dec;20(6):224

More than one antibody of individual B cells revealed by single-cell immune profiling.

Shi Z, Zhang Q, Yan H, Yang Y, Wang P, Zhang Y, Deng Z, Yu M, Zhou W, Wang Q, Yang X, Mo X, Zhang C, Huang J, Dai H, Sun B, Zhao Y, Zhang L, Yang YG, Qiu X
Cell discovery 2019;5:64

Perturbed CD8(+) T cell immunity across universal influenza epitopes in the elderly.

Nguyen THO, Sant S, Bird NL, Grant EJ, Clemens EB, Koutsakos M, Valkenburg SA, Gras S, Lappas M, Jaworowski A, Crowe J, Loh L, Kedzierska K
Journal of leukocyte biology 2018 Feb;103(2):321-339

Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.

Van Caeneghem Y, De Munter S, Tieppo P, Goetgeluk G, Weening K, Verstichel G, Bonte S, Taghon T, Leclercq G, Kerre T, Debets R, Vermijlen D, Abken H, Vandekerckhove B
Oncoimmunology 2017;6(3):e1283460

Targeting CD22 with the monoclonal antibody epratuzumab modulates human B-cell maturation and cytokine production in response to Toll-like receptor 7 (TLR7) and B-cell receptor (BCR) signaling.

Giltiay NV, Shu GL, Shock A, Clark EA
Arthritis research & therapy 2017 May 15;19(1):91

Maintenance of the EBV-specific CD8(+) TCRαβ repertoire in immunosuppressed lung transplant recipients.

Nguyen TH, Bird NL, Grant EJ, Miles JJ, Thomas PG, Kotsimbos TC, Mifsud NA, Kedzierska K
Immunology and cell biology 2017 Jan;95(1):77-86

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Kong F, Feng B, Zhang H, Rao H, Wang J, Cong X, Wei L
Experimental and therapeutic medicine 2017 Aug;14(2):1846-1852

Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.

Ndungu FM, Lundblom K, Rono J, Illingworth J, Eriksson S, Färnert A
European journal of immunology 2013 Nov;43(11):2919-29

Humanized mice, a new model to study the influence of drug treatment on neonatal sepsis.

Ernst W, Zimara N, Hanses F, Männel DN, Seelbach-Göbel B, Wege AK
Infection and immunity 2013 May;81(5):1520-31

Atypical and classical memory B cells produce Plasmodium falciparum neutralizing antibodies.

Muellenbeck MF, Ueberheide B, Amulic B, Epp A, Fenyo D, Busse CE, Esen M, Theisen M, Mordmüller B, Wardemann H
The Journal of experimental medicine 2013 Feb 11;210(2):389-99

Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.

Illingworth J, Butler NS, Roetynck S, Mwacharo J, Pierce SK, Bejon P, Crompton PD, Marsh K, Ndungu FM
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47

Leishmania major infection in humanized mice induces systemic infection and provokes a nonprotective human immune response.

Wege AK, Florian C, Ernst W, Zimara N, Schleicher U, Hanses F, Schmid M, Ritter U
PLoS neglected tropical diseases 2012;6(7):e1741

Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.

Kuzin I, Sun H, Moshkani S, Feng C, Mantalaris A, Wu JH, Bottaro A
Biotechnology and bioengineering 2011 Jun;108(6):1430-40

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Staining of normal human peripheral blood cells with Anti-Human CD27 PE (left), and APC (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Early detection of TRAV5-biased GLC-specific CD8 + T cells in patient Tx101. PBMC from patient Tx101 ( a ) at 87 days post transplant underwent tetramer-associated magnetic enrichment (TAME). Pre-enriched, post-enriched and the flowthrough fractions were collected and analyzed based on GLC-tetramer and anti-CD8 antibody staining. Percentages are based on CD3 + T cells. CD3 + T cells, GLC-tetramer + CD8 + and GLC-tetramer + CD8 low/- cells from the post-enriched fraction ( b ) were phenotypically analyzed based on CD45RA and CD27 expression. Pie charts of paired GLC-specific CD8 + TCRalphabeta clonotypes following TAME (d87) ( c ) or following in vitro expansion (d185, d299 and d472) ( d ) are shown. Pie chart area shaded in red represent TRAV5 clonotypes. Percentage of TCRalphabeta clonotypes shared compared to TAME (d87) (bar graph, left y axis) and SDI values (line graph, right y axis) are shown in e . Frequencies of GLC + CD8 + T cells following expansion and clonotype details are listed in f (ND; not determined).
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 Sanger sequencing validation of multiple V(D)J recombination patterns in single B cells. a Sketching diagram of the single-cell sequencing procedure. We isolated peripheral blood from healthy donors and separated peripheral blood mononuclear cells (PBMCs). Naive B cells (CD19 + CD38 +/- CD10 - CD27 - ), memory B cells (CD19 + CD38 +/- CD10 - CD27 + ), and plasma cells (CD19 + CD38 +++ CD10 - ) were sorted by FACS. The total mRNA in single B cells was reverse transcribed, and the Ig heavy chain and light chain were amplified by multiplex PCR. FACS, fluorescence-activated cell sorting. b Proportions of cells expressing VDJ recombination patterns in single B cells from donor 7, donor 8, and donor 9. c - e Distribution and frequency of IGHV ( c ), IGHD ( d ), and IGHJ ( e ) segments in Ig germline genes in a single cell. f Proportions of single naive B cells, plasma cells, and memory B cells expressing one, two, or three V H DJ H segments. g , h Proportions of single B cells expressing one, two, or more V kappa J kappa segments ( g ) and V lamda J lamda segments ( h ). i Counts of single B cells expressing only Igkappa or Iglamda or expressing both Igkappa and Iglamda. j , k Proportions of naive B cells, plasma cells, and memory B cells expressing one, two, three, or four Ig classes
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Expression pattern of CD27 and CD28 on gammadelta T cells in AR. (A) Representative flow cytometry plots of the expression of CD27 and CD28 on gammadelta T cells among PBMCs from HC (n=12) and AR (n=14) subjects. (B-G) Quantification of the percentage of (B) CD27+, (C) CD28+, (D) CD27 + CD28 + , (E) CD27 + CD28 - , (F) CD27 - CD28 + and (G) CD27 - CD28 - gammadelta + T cells in PBMCs. Values are expressed as the mean +- standard deviation. AR, allergic rhinitis; HC, healthy controls; PBMCs, peripheral blood mononuclear cells.
Conjugate: Biotin

13-0279-82

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