Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-0272-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD27 Monoclonal Antibody (LG.3A10), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The LG.3A10 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.8F10 cross-reacts with human and rat CD27.
- Antibody clone number
- LG.3A10
- Concentration
- 0.5 mg/mL
Submitted references NFκB-Pim-1-Eomesodermin axis is critical for maintaining CD8 T-cell memory quality.
Direct tumor recognition by a human CD4(+) T-cell subset potently mediates tumor growth inhibition and orchestrates anti-tumor immune responses.
Inflammation enhances IL-2 driven differentiation of cytolytic CD4 T cells.
CD70 is downregulated by interaction with CD27.
CD70-driven costimulation induces survival or Fas-mediated apoptosis of T cells depending on antigenic load.
Sequential desensitization of CXCR4 and S1P5 controls natural killer cell trafficking.
Rescue of tumor-infiltrating lymphocytes from activation-induced cell death enhances the antitumor CTL response in CD5-deficient mice.
Chronic CD70-driven costimulation impairs IgG responses by instructing T cells to inhibit germinal center B cell formation through FasL-Fas interactions.
Knudson KM, Pritzl CJ, Saxena V, Altman A, Daniels MA, Teixeiro E
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 28;114(9):E1659-E1667
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 28;114(9):E1659-E1667
Direct tumor recognition by a human CD4(+) T-cell subset potently mediates tumor growth inhibition and orchestrates anti-tumor immune responses.
Matsuzaki J, Tsuji T, Luescher IF, Shiku H, Mineno J, Okamoto S, Old LJ, Shrikant P, Gnjatic S, Odunsi K
Scientific reports 2015 Oct 8;5:14896
Scientific reports 2015 Oct 8;5:14896
Inflammation enhances IL-2 driven differentiation of cytolytic CD4 T cells.
Workman AM, Jacobs AK, Vogel AJ, Condon S, Brown DM
PloS one 2014;9(2):e89010
PloS one 2014;9(2):e89010
CD70 is downregulated by interaction with CD27.
Kuka M, Munitic I, Giardino Torchia ML, Ashwell JD
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 1;191(5):2282-9
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 1;191(5):2282-9
CD70-driven costimulation induces survival or Fas-mediated apoptosis of T cells depending on antigenic load.
Wensveen FM, Unger PP, Kragten NA, Derks IA, ten Brinke A, Arens R, van Lier RA, Eldering E, van Gisbergen KP
Journal of immunology (Baltimore, Md. : 1950) 2012 May 1;188(9):4256-67
Journal of immunology (Baltimore, Md. : 1950) 2012 May 1;188(9):4256-67
Sequential desensitization of CXCR4 and S1P5 controls natural killer cell trafficking.
Mayol K, Biajoux V, Marvel J, Balabanian K, Walzer T
Blood 2011 Nov 3;118(18):4863-71
Blood 2011 Nov 3;118(18):4863-71
Rescue of tumor-infiltrating lymphocytes from activation-induced cell death enhances the antitumor CTL response in CD5-deficient mice.
Tabbekh M, Franciszkiewicz K, Haouas H, Lécluse Y, Benihoud K, Raman C, Mami-Chouaib F
Journal of immunology (Baltimore, Md. : 1950) 2011 Jul 1;187(1):102-9
Journal of immunology (Baltimore, Md. : 1950) 2011 Jul 1;187(1):102-9
Chronic CD70-driven costimulation impairs IgG responses by instructing T cells to inhibit germinal center B cell formation through FasL-Fas interactions.
Beishuizen CR, Kragten NA, Boon L, Nolte MA, van Lier RA, van Gisbergen KP
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 15;183(10):6442-51
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 15;183(10):6442-51
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg Anti-CD27 Purified followed by Anti-Armenian Hamster IgG PE (Product # 12-4112-83). Total viable cells were analyzed.