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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [47]
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- Validations
- Flow cytometry [1]
- Other assay [13]
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- Product number
- 17-0279-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD27 Monoclonal Antibody (O323), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction. Applications Reported: The O323 antibody has been reported for use in flow cytometric analysis. Applications Tested: This O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- O323
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Mucosal-associated invariant T cell responses differ by sex in COVID-19.
Antibody Feedback Limits the Expansion of B Cell Responses to Malaria Vaccination but Drives Diversification of the Humoral Response.
Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.
More than one antibody of individual B cells revealed by single-cell immune profiling.
Breadth and Functionality of Varicella-Zoster Virus Glycoprotein-Specific Antibodies Identified after Zostavax Vaccination in Humans.
Perturbed CD8(+) T cell immunity across universal influenza epitopes in the elderly.
CD4 T-Cell Dysregulation in Psoriatic Arthritis Reveals a Regulatory Role for IL-22.
Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.
Distinctive features of tumor-infiltrating γδ T lymphocytes in human colorectal cancer.
Clonal relationships of CSF B cells in treatment-naive multiple sclerosis patients.
Squamous Cell Tumors Recruit γδ T Cells Producing either IL17 or IFNγ Depending on the Tumor Stage.
Maintenance of the EBV-specific CD8(+) TCRαβ repertoire in immunosuppressed lung transplant recipients.
Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.
CD16(+) Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.
A distinct plasmablast and naïve B-cell phenotype in primary immune thrombocytopenia.
B cells from African American lupus patients exhibit an activated phenotype.
An epigenetic clock analysis of race/ethnicity, sex, and coronary heart disease.
Delta Inulin Adjuvant Enhances Plasmablast Generation, Expression of Activation-Induced Cytidine Deaminase and B-Cell Affinity Maturation in Human Subjects Receiving Seasonal Influenza Vaccine.
Exploring the feasibility of multi-site flow cytometric processing of gut associated lymphoid tissue with centralized data analysis for multi-site clinical trials.
HIV Malaria Co-Infection Is Associated with Atypical Memory B Cell Expansion and a Reduced Antibody Response to a Broad Array of Plasmodium falciparum Antigens in Rwandan Adults.
Acceleration of age-associated methylation patterns in HIV-1-infected adults.
Somatic Variation of T-Cell Receptor Genes Strongly Associate with HLA Class Restriction.
Human HOIP and LUBAC deficiency underlies autoinflammation, immunodeficiency, amylopectinosis, and lymphangiectasia.
DCLRE1C (ARTEMIS) mutations causing phenotypes ranging from atypical severe combined immunodeficiency to mere antibody deficiency.
Accumulation of self-reactive naïve and memory B cell reveals sequential defects in B cell tolerance checkpoints in Sjögren's syndrome.
Metabolic engineering of Salmonella vaccine bacteria to boost human Vγ2Vδ2 T cell immunity.
9G4+ antibodies isolated from HIV-infected patients neutralize HIV-1 and have distinct autoreactivity profiles.
Changes of cytokines during a spaceflight analog--a 45-day head-down bed rest.
Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.
Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.
Signal transducer and activator of transcription 3 (STAT3) mutations underlying autosomal dominant hyper-IgE syndrome impair human CD8(+) T-cell memory formation and function.
Measles immune suppression: lessons from the macaque model.
Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions.
The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
Impact of persistent HIV replication on CD4 negative Vγ2Vδ2 T cells.
Rapid and massive virus-specific plasmablast responses during acute dengue virus infection in humans.
Vigorous response of human innate functioning IgM memory B cells upon infection by Neisseria gonorrhoeae.
Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection.
DOCK8 deficiency impairs CD8 T cell survival and function in humans and mice.
Differential expression of CD300a/c on human TH1 and TH17 cells.
Altered dynamics and differential infection profiles of lymphoid and myeloid cell subsets during acute and chronic HIV-1 infection.
Alterations in peripheral blood B-cell populations in SHIV89.6P-infected macaques (Macacca fascicularis).
Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.
Alterations on peripheral blood B-cell subpopulations in very early arthritis patients.
Cytokine requirements for the differentiation and expansion of IL-17A- and IL-22-producing human Vgamma2Vdelta2 T cells.
Natural viral suppressors of HIV-1 have a unique capacity to maintain gammadelta T cells.
Impacts of HIV infection on Vgamma2Vdelta2 T cell phenotype and function: a mechanism for reduced tumor immunity in AIDS.
Yu C, Littleton S, Giroux NS, Mathew R, Ding S, Kalnitsky J, Yang Y, Petzold E, Chung HA, Rivera GO, Rotstein T, Xi R, Ko ER, Tsalik EL, Sempowski GD, Denny TN, Burke TW, McClain MT, Woods CW, Shen X, Saban DR
Med (New York, N.Y.) 2021 Jun 11;2(6):755-772.e5
Med (New York, N.Y.) 2021 Jun 11;2(6):755-772.e5
Antibody Feedback Limits the Expansion of B Cell Responses to Malaria Vaccination but Drives Diversification of the Humoral Response.
McNamara HA, Idris AH, Sutton HJ, Vistein R, Flynn BJ, Cai Y, Wiehe K, Lyke KE, Chatterjee D, Kc N, Chakravarty S, Lee Sim BK, Hoffman SL, Bonsignori M, Seder RA, Cockburn IA
Cell host & microbe 2020 Oct 7;28(4):572-585.e7
Cell host & microbe 2020 Oct 7;28(4):572-585.e7
Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.
Wang Q, Sun Q, Chen Q, Li H, Liu D
Experimental and therapeutic medicine 2020 Dec;20(6):224
Experimental and therapeutic medicine 2020 Dec;20(6):224
More than one antibody of individual B cells revealed by single-cell immune profiling.
Shi Z, Zhang Q, Yan H, Yang Y, Wang P, Zhang Y, Deng Z, Yu M, Zhou W, Wang Q, Yang X, Mo X, Zhang C, Huang J, Dai H, Sun B, Zhao Y, Zhang L, Yang YG, Qiu X
Cell discovery 2019;5:64
Cell discovery 2019;5:64
Breadth and Functionality of Varicella-Zoster Virus Glycoprotein-Specific Antibodies Identified after Zostavax Vaccination in Humans.
Sullivan NL, Reuter-Monslow MA, Sei J, Durr E, Davis CW, Chang C, McCausland M, Wieland A, Krah D, Rouphael N, Mehta AK, Mulligan MJ, Pulendran B, Ahmed R, Vora KA
Journal of virology 2018 Jul 15;92(14)
Journal of virology 2018 Jul 15;92(14)
Perturbed CD8(+) T cell immunity across universal influenza epitopes in the elderly.
Nguyen THO, Sant S, Bird NL, Grant EJ, Clemens EB, Koutsakos M, Valkenburg SA, Gras S, Lappas M, Jaworowski A, Crowe J, Loh L, Kedzierska K
Journal of leukocyte biology 2018 Feb;103(2):321-339
Journal of leukocyte biology 2018 Feb;103(2):321-339
CD4 T-Cell Dysregulation in Psoriatic Arthritis Reveals a Regulatory Role for IL-22.
Ezeonyeji A, Baldwin H, Vukmanovic-Stejic M, Ehrenstein MR
Frontiers in immunology 2017;8:1403
Frontiers in immunology 2017;8:1403
Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.
Van Caeneghem Y, De Munter S, Tieppo P, Goetgeluk G, Weening K, Verstichel G, Bonte S, Taghon T, Leclercq G, Kerre T, Debets R, Vermijlen D, Abken H, Vandekerckhove B
Oncoimmunology 2017;6(3):e1283460
Oncoimmunology 2017;6(3):e1283460
Distinctive features of tumor-infiltrating γδ T lymphocytes in human colorectal cancer.
Meraviglia S, Lo Presti E, Tosolini M, La Mendola C, Orlando V, Todaro M, Catalano V, Stassi G, Cicero G, Vieni S, Fourniè JJ, Dieli F
Oncoimmunology 2017;6(10):e1347742
Oncoimmunology 2017;6(10):e1347742
Clonal relationships of CSF B cells in treatment-naive multiple sclerosis patients.
Eggers EL, Michel BA, Wu H, Wang SZ, Bevan CJ, Abounasr A, Pierson NS, Bischof A, Kazer M, Leitner E, Greenfield AL, Demuth S, Wilson MR, Henry RG, Cree BA, Hauser SL, von Büdingen HC
JCI insight 2017 Nov 16;2(22)
JCI insight 2017 Nov 16;2(22)
Squamous Cell Tumors Recruit γδ T Cells Producing either IL17 or IFNγ Depending on the Tumor Stage.
Lo Presti E, Toia F, Oieni S, Buccheri S, Turdo A, Mangiapane LR, Campisi G, Caputo V, Todaro M, Stassi G, Cordova A, Moschella F, Rinaldi G, Meraviglia S, Dieli F
Cancer immunology research 2017 May;5(5):397-407
Cancer immunology research 2017 May;5(5):397-407
Maintenance of the EBV-specific CD8(+) TCRαβ repertoire in immunosuppressed lung transplant recipients.
Nguyen TH, Bird NL, Grant EJ, Miles JJ, Thomas PG, Kotsimbos TC, Mifsud NA, Kedzierska K
Immunology and cell biology 2017 Jan;95(1):77-86
Immunology and cell biology 2017 Jan;95(1):77-86
Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.
Kong F, Feng B, Zhang H, Rao H, Wang J, Cong X, Wei L
Experimental and therapeutic medicine 2017 Aug;14(2):1846-1852
Experimental and therapeutic medicine 2017 Aug;14(2):1846-1852
CD16(+) Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.
Zhu H, Hu F, Sun X, Zhang X, Zhu L, Liu X, Li X, Xu L, Shi L, Gan Y, Su Y
Frontiers in immunology 2016;7:512
Frontiers in immunology 2016;7:512
A distinct plasmablast and naïve B-cell phenotype in primary immune thrombocytopenia.
Flint SM, Gibson A, Lucas G, Nandigam R, Taylor L, Provan D, Newland AC, Savage CO, Henderson RB
Haematologica 2016 Jun;101(6):698-706
Haematologica 2016 Jun;101(6):698-706
B cells from African American lupus patients exhibit an activated phenotype.
Menard LC, Habte S, Gonsiorek W, Lee D, Banas D, Holloway DA, Manjarrez-Orduno N, Cunningham M, Stetsko D, Casano F, Kansal S, Davis PM, Carman J, Zhang CK, Abidi F, Furie R, Nadler SG, Suchard SJ
JCI insight 2016 Jun 16;1(9):e87310
JCI insight 2016 Jun 16;1(9):e87310
An epigenetic clock analysis of race/ethnicity, sex, and coronary heart disease.
Horvath S, Gurven M, Levine ME, Trumble BC, Kaplan H, Allayee H, Ritz BR, Chen B, Lu AT, Rickabaugh TM, Jamieson BD, Sun D, Li S, Chen W, Quintana-Murci L, Fagny M, Kobor MS, Tsao PS, Reiner AP, Edlefsen KL, Absher D, Assimes TL
Genome biology 2016 Aug 11;17(1):171
Genome biology 2016 Aug 11;17(1):171
Delta Inulin Adjuvant Enhances Plasmablast Generation, Expression of Activation-Induced Cytidine Deaminase and B-Cell Affinity Maturation in Human Subjects Receiving Seasonal Influenza Vaccine.
Li L, Honda-Okubo Y, Li C, Sajkov D, Petrovsky N
PloS one 2015;10(7):e0132003
PloS one 2015;10(7):e0132003
Exploring the feasibility of multi-site flow cytometric processing of gut associated lymphoid tissue with centralized data analysis for multi-site clinical trials.
McGowan I, Anton PA, Elliott J, Cranston RD, Duffill K, Althouse AD, Hawkins KL, De Rosa SC
PloS one 2015;10(5):e0126454
PloS one 2015;10(5):e0126454
HIV Malaria Co-Infection Is Associated with Atypical Memory B Cell Expansion and a Reduced Antibody Response to a Broad Array of Plasmodium falciparum Antigens in Rwandan Adults.
Subramaniam KS, Skinner J, Ivan E, Mutimura E, Kim RS, Feintuch CM, Portugal S, Anastos K, Crompton PD, Daily JP
PloS one 2015;10(4):e0124412
PloS one 2015;10(4):e0124412
Acceleration of age-associated methylation patterns in HIV-1-infected adults.
Rickabaugh TM, Baxter RM, Sehl M, Sinsheimer JS, Hultin PM, Hultin LE, Quach A, Martínez-Maza O, Horvath S, Vilain E, Jamieson BD
PloS one 2015;10(3):e0119201
PloS one 2015;10(3):e0119201
Somatic Variation of T-Cell Receptor Genes Strongly Associate with HLA Class Restriction.
Klarenbeek PL, Doorenspleet ME, Esveldt RE, van Schaik BD, Lardy N, van Kampen AH, Tak PP, Plenge RM, Baas F, de Bakker PI, de Vries N
PloS one 2015;10(10):e0140815
PloS one 2015;10(10):e0140815
Human HOIP and LUBAC deficiency underlies autoinflammation, immunodeficiency, amylopectinosis, and lymphangiectasia.
Boisson B, Laplantine E, Dobbs K, Cobat A, Tarantino N, Hazen M, Lidov HG, Hopkins G, Du L, Belkadi A, Chrabieh M, Itan Y, Picard C, Fournet JC, Eibel H, Tsitsikov E, Pai SY, Abel L, Al-Herz W, Casanova JL, Israel A, Notarangelo LD
The Journal of experimental medicine 2015 Jun 1;212(6):939-51
The Journal of experimental medicine 2015 Jun 1;212(6):939-51
DCLRE1C (ARTEMIS) mutations causing phenotypes ranging from atypical severe combined immunodeficiency to mere antibody deficiency.
Volk T, Pannicke U, Reisli I, Bulashevska A, Ritter J, Björkman A, Schäffer AA, Fliegauf M, Sayar EH, Salzer U, Fisch P, Pfeifer D, Di Virgilio M, Cao H, Yang F, Zimmermann K, Keles S, Caliskaner Z, Güner SÜ, Schindler D, Hammarström L, Rizzi M, Hummel M, Pan-Hammarström Q, Schwarz K, Grimbacher B
Human molecular genetics 2015 Dec 20;24(25):7361-72
Human molecular genetics 2015 Dec 20;24(25):7361-72
Accumulation of self-reactive naïve and memory B cell reveals sequential defects in B cell tolerance checkpoints in Sjögren's syndrome.
Corsiero E, Sutcliffe N, Pitzalis C, Bombardieri M
PloS one 2014;9(12):e114575
PloS one 2014;9(12):e114575
Metabolic engineering of Salmonella vaccine bacteria to boost human Vγ2Vδ2 T cell immunity.
Workalemahu G, Wang H, Puan KJ, Nada MH, Kuzuyama T, Jones BD, Jin C, Morita CT
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 15;193(2):708-21
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 15;193(2):708-21
9G4+ antibodies isolated from HIV-infected patients neutralize HIV-1 and have distinct autoreactivity profiles.
Alcéna DC, Kobie JJ, Kaminski DA, Rosenberg AF, Mattiacio JL, Brewer M, Dewhurst S, Dykes C, Jin X, Keefer MC, Sanz I
PloS one 2013;8(12):e85098
PloS one 2013;8(12):e85098
Changes of cytokines during a spaceflight analog--a 45-day head-down bed rest.
Xu X, Tan C, Li P, Zhang S, Pang X, Liu H, Li L, Sun X, Zhang Y, Wu H, Chen X, Ge Q
PloS one 2013;8(10):e77401
PloS one 2013;8(10):e77401
Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.
Ndungu FM, Lundblom K, Rono J, Illingworth J, Eriksson S, Färnert A
European journal of immunology 2013 Nov;43(11):2919-29
European journal of immunology 2013 Nov;43(11):2919-29
Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.
Illingworth J, Butler NS, Roetynck S, Mwacharo J, Pierce SK, Bejon P, Crompton PD, Marsh K, Ndungu FM
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
Signal transducer and activator of transcription 3 (STAT3) mutations underlying autosomal dominant hyper-IgE syndrome impair human CD8(+) T-cell memory formation and function.
Ives ML, Ma CS, Palendira U, Chan A, Bustamante J, Boisson-Dupuis S, Arkwright PD, Engelhard D, Averbuch D, Magdorf K, Roesler J, Peake J, Wong M, Adelstein S, Choo S, Smart JM, French MA, Fulcher DA, Cook MC, Picard C, Durandy A, Tsumura M, Kobayashi M, Uzel G, Casanova JL, Tangye SG, Deenick EK
The Journal of allergy and clinical immunology 2013 Aug;132(2):400-11.e9
The Journal of allergy and clinical immunology 2013 Aug;132(2):400-11.e9
Measles immune suppression: lessons from the macaque model.
de Vries RD, McQuaid S, van Amerongen G, Yüksel S, Verburgh RJ, Osterhaus AD, Duprex WP, de Swart RL
PLoS pathogens 2012;8(8):e1002885
PLoS pathogens 2012;8(8):e1002885
Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions.
Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW
PLoS pathogens 2012;8(11):e1002984
PLoS pathogens 2012;8(11):e1002984
The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
Slyker JA, Lohman-Payne B, John-Stewart GC, Dong T, Mbori-Ngacha D, Tapia K, Atzberger A, Taylor S, Rowland-Jones SL, Blish CA
Frontiers in immunology 2012;3:399
Frontiers in immunology 2012;3:399
Impact of persistent HIV replication on CD4 negative Vγ2Vδ2 T cells.
Boudová S, Li H, Sajadi MM, Redfield RR, Pauza CD
The Journal of infectious diseases 2012 May 1;205(9):1448-55
The Journal of infectious diseases 2012 May 1;205(9):1448-55
Rapid and massive virus-specific plasmablast responses during acute dengue virus infection in humans.
Wrammert J, Onlamoon N, Akondy RS, Perng GC, Polsrila K, Chandele A, Kwissa M, Pulendran B, Wilson PC, Wittawatmongkol O, Yoksan S, Angkasekwinai N, Pattanapanyasat K, Chokephaibulkit K, Ahmed R
Journal of virology 2012 Mar;86(6):2911-8
Journal of virology 2012 Mar;86(6):2911-8
Vigorous response of human innate functioning IgM memory B cells upon infection by Neisseria gonorrhoeae.
So NS, Ostrowski MA, Gray-Owen SD
Journal of immunology (Baltimore, Md. : 1950) 2012 Apr 15;188(8):4008-22
Journal of immunology (Baltimore, Md. : 1950) 2012 Apr 15;188(8):4008-22
Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection.
Slyker JA, John-Stewart GC, Dong T, Lohman-Payne B, Reilly M, Atzberger A, Taylor S, Maleche-Obimbo E, Mbori-Ngacha D, Rowland-Jones SL
PloS one 2011;6(5):e20375
PloS one 2011;6(5):e20375
DOCK8 deficiency impairs CD8 T cell survival and function in humans and mice.
Randall KL, Chan SS, Ma CS, Fung I, Mei Y, Yabas M, Tan A, Arkwright PD, Al Suwairi W, Lugo Reyes SO, Yamazaki-Nakashimada MA, Garcia-Cruz Mde L, Smart JM, Picard C, Okada S, Jouanguy E, Casanova JL, Lambe T, Cornall RJ, Russell S, Oliaro J, Tangye SG, Bertram EM, Goodnow CC
The Journal of experimental medicine 2011 Oct 24;208(11):2305-20
The Journal of experimental medicine 2011 Oct 24;208(11):2305-20
Differential expression of CD300a/c on human TH1 and TH17 cells.
Simhadri VR, Mariano JL, Zhou Q, DeBell KE, Borrego F
BMC immunology 2011 Nov 2;12:62
BMC immunology 2011 Nov 2;12:62
Altered dynamics and differential infection profiles of lymphoid and myeloid cell subsets during acute and chronic HIV-1 infection.
Centlivre M, Legrand N, Steingrover R, van der Sluis R, Grijsen ML, Bakker M, Jurriaans S, Berkhout B, Paxton WA, Prins JM, Pollakis G
Journal of leukocyte biology 2011 May;89(5):785-95
Journal of leukocyte biology 2011 May;89(5):785-95
Alterations in peripheral blood B-cell populations in SHIV89.6P-infected macaques (Macacca fascicularis).
Kling HM, Shipley TW, Norris KA
Comparative medicine 2011 Jun;61(3):269-77
Comparative medicine 2011 Jun;61(3):269-77
Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.
Kuzin I, Sun H, Moshkani S, Feng C, Mantalaris A, Wu JH, Bottaro A
Biotechnology and bioengineering 2011 Jun;108(6):1430-40
Biotechnology and bioengineering 2011 Jun;108(6):1430-40
Alterations on peripheral blood B-cell subpopulations in very early arthritis patients.
Moura RA, Weinmann P, Pereira PA, Caetano-Lopes J, Canhão H, Sousa E, Mourão AF, Rodrigues AM, Queiroz MV, Souto-Carneiro MM, Graça L, Fonseca JE
Rheumatology (Oxford, England) 2010 Jun;49(6):1082-92
Rheumatology (Oxford, England) 2010 Jun;49(6):1082-92
Cytokine requirements for the differentiation and expansion of IL-17A- and IL-22-producing human Vgamma2Vdelta2 T cells.
Ness-Schwickerath KJ, Jin C, Morita CT
Journal of immunology (Baltimore, Md. : 1950) 2010 Jun 15;184(12):7268-80
Journal of immunology (Baltimore, Md. : 1950) 2010 Jun 15;184(12):7268-80
Natural viral suppressors of HIV-1 have a unique capacity to maintain gammadelta T cells.
Riedel DJ, Sajadi MM, Armstrong CL, Cummings JS, Cairo C, Redfield RR, Pauza CD
AIDS (London, England) 2009 Sep 24;23(15):1955-64
AIDS (London, England) 2009 Sep 24;23(15):1955-64
Impacts of HIV infection on Vgamma2Vdelta2 T cell phenotype and function: a mechanism for reduced tumor immunity in AIDS.
Cummings JS, Cairo C, Armstrong C, Davis CE, Pauza CD
Journal of leukocyte biology 2008 Aug;84(2):371-9
Journal of leukocyte biology 2008 Aug;84(2):371-9
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Supportive validation
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- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (open histogram) or Anti-Human CD27 APC (filled histogram). Cells in the lymphocyte gate were used for analysis.
Supportive validation
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- Figure 4 Early detection of TRAV5-biased GLC-specific CD8 + T cells in patient Tx101. PBMC from patient Tx101 ( a ) at 87 days post transplant underwent tetramer-associated magnetic enrichment (TAME). Pre-enriched, post-enriched and the flowthrough fractions were collected and analyzed based on GLC-tetramer and anti-CD8 antibody staining. Percentages are based on CD3 + T cells. CD3 + T cells, GLC-tetramer + CD8 + and GLC-tetramer + CD8 low/- cells from the post-enriched fraction ( b ) were phenotypically analyzed based on CD45RA and CD27 expression. Pie charts of paired GLC-specific CD8 + TCRalphabeta clonotypes following TAME (d87) ( c ) or following in vitro expansion (d185, d299 and d472) ( d ) are shown. Pie chart area shaded in red represent TRAV5 clonotypes. Percentage of TCRalphabeta clonotypes shared compared to TAME (d87) (bar graph, left y axis) and SDI values (line graph, right y axis) are shown in e . Frequencies of GLC + CD8 + T cells following expansion and clonotype details are listed in f (ND; not determined).
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- Fig. 4 Sanger sequencing validation of multiple V(D)J recombination patterns in single B cells. a Sketching diagram of the single-cell sequencing procedure. We isolated peripheral blood from healthy donors and separated peripheral blood mononuclear cells (PBMCs). Naive B cells (CD19 + CD38 +/- CD10 - CD27 - ), memory B cells (CD19 + CD38 +/- CD10 - CD27 + ), and plasma cells (CD19 + CD38 +++ CD10 - ) were sorted by FACS. The total mRNA in single B cells was reverse transcribed, and the Ig heavy chain and light chain were amplified by multiplex PCR. FACS, fluorescence-activated cell sorting. b Proportions of cells expressing VDJ recombination patterns in single B cells from donor 7, donor 8, and donor 9. c - e Distribution and frequency of IGHV ( c ), IGHD ( d ), and IGHJ ( e ) segments in Ig germline genes in a single cell. f Proportions of single naive B cells, plasma cells, and memory B cells expressing one, two, or three V H DJ H segments. g , h Proportions of single B cells expressing one, two, or more V kappa J kappa segments ( g ) and V lamda J lamda segments ( h ). i Counts of single B cells expressing only Igkappa or Iglamda or expressing both Igkappa and Iglamda. j , k Proportions of naive B cells, plasma cells, and memory B cells expressing one, two, three, or four Ig classes
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- Figure 3 Expression pattern of CD27 and CD28 on gammadelta T cells in AR. (A) Representative flow cytometry plots of the expression of CD27 and CD28 on gammadelta T cells among PBMCs from HC (n=12) and AR (n=14) subjects. (B-G) Quantification of the percentage of (B) CD27+, (C) CD28+, (D) CD27 + CD28 + , (E) CD27 + CD28 - , (F) CD27 - CD28 + and (G) CD27 - CD28 - gammadelta + T cells in PBMCs. Values are expressed as the mean +- standard deviation. AR, allergic rhinitis; HC, healthy controls; PBMCs, peripheral blood mononuclear cells.
