Antibody data
- Antibody Data
- Antigen structure
- References [25]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [13]
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- Product number
- 25-0279-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD27 Monoclonal Antibody (O323), PE-Cyanine7, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction. Applications Reported: This O323 antibody has been reported for use in flow cytometric analysis. Applications Tested: This O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- O323
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Dynamics of NK, CD8 and Tfh cell mediated the production of cytokines and antiviral antibodies in Chinese patients with moderate COVID-19.
Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.
More than one antibody of individual B cells revealed by single-cell immune profiling.
CD19(+)CD24(hi)CD38(hi) B Cells Are Expanded in Juvenile Dermatomyositis and Exhibit a Pro-Inflammatory Phenotype After Activation Through Toll-Like Receptor 7 and Interferon-α.
Perturbed CD8(+) T cell immunity across universal influenza epitopes in the elderly.
Clonally diverse CD38(+)HLA-DR(+)CD8(+) T cells persist during fatal H7N9 disease.
Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.
Maintenance of the EBV-specific CD8(+) TCRαβ repertoire in immunosuppressed lung transplant recipients.
High-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine.
IL-15 enhances the anti-tumor activity of trastuzumab against breast cancer cells but causes fatal side effects in humanized tumor mice (HTM).
Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.
Reduced expression of CD27 by collagenase treatment: implications for interpreting b cell data in tissues.
Rapid Development of gp120-Focused Neutralizing B Cell Responses during Acute Simian Immunodeficiency Virus Infection of African Green Monkeys.
Restricted isotype, distinct variable gene usage, and high rate of gp120 specificity of HIV-1 envelope-specific B cells in colostrum compared with those in blood of HIV-1-infected, lactating African women.
Phenotypic, genomic and functional characterization reveals no differences between CD138++ and CD138low subpopulations in multiple myeloma cell lines.
Adipose tissue NK cells manifest an activated phenotype in human obesity.
Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.
Long-term immune reconstitution of naive and memory t cell pools after haploidentical hematopoietic stem cell transplantation.
Rabbit antithymocyte globulin (thymoglobulin) impairs the thymic output of both conventional and regulatory CD4+ T cells after allogeneic hematopoietic stem cell transplantation in adult patients.
Follicular helper T cells serve as the major CD4 T cell compartment for HIV-1 infection, replication, and production.
Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.
B cell analysis of ethnic groups in Mali with differential susceptibility to malaria.
Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.
Vaccine-elicited SIV and HIV envelope-specific IgA and IgG memory B cells in rhesus macaque peripheral blood correlate with functional antibody responses and reduced viremia.
Chronic lymphocytic leukaemia (CLL) and CLL-type monoclonal B-cell lymphocytosis (MBL) show differential expression of molecules involved in lymphoid tissue homing.
Yan L, Cai B, Li Y, Wang MJ, An YF, Deng R, Li DD, Wang LC, Xu H, Gao XD, Wang LL
Journal of cellular and molecular medicine 2020 Dec;24(24):14270-14279
Journal of cellular and molecular medicine 2020 Dec;24(24):14270-14279
Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.
Wang Q, Sun Q, Chen Q, Li H, Liu D
Experimental and therapeutic medicine 2020 Dec;20(6):224
Experimental and therapeutic medicine 2020 Dec;20(6):224
More than one antibody of individual B cells revealed by single-cell immune profiling.
Shi Z, Zhang Q, Yan H, Yang Y, Wang P, Zhang Y, Deng Z, Yu M, Zhou W, Wang Q, Yang X, Mo X, Zhang C, Huang J, Dai H, Sun B, Zhao Y, Zhang L, Yang YG, Qiu X
Cell discovery 2019;5:64
Cell discovery 2019;5:64
CD19(+)CD24(hi)CD38(hi) B Cells Are Expanded in Juvenile Dermatomyositis and Exhibit a Pro-Inflammatory Phenotype After Activation Through Toll-Like Receptor 7 and Interferon-α.
Piper CJM, Wilkinson MGL, Deakin CT, Otto GW, Dowle S, Duurland CL, Adams S, Marasco E, Rosser EC, Radziszewska A, Carsetti R, Ioannou Y, Beales PL, Kelberman D, Isenberg DA, Mauri C, Nistala K, Wedderburn LR
Frontiers in immunology 2018;9:1372
Frontiers in immunology 2018;9:1372
Perturbed CD8(+) T cell immunity across universal influenza epitopes in the elderly.
Nguyen THO, Sant S, Bird NL, Grant EJ, Clemens EB, Koutsakos M, Valkenburg SA, Gras S, Lappas M, Jaworowski A, Crowe J, Loh L, Kedzierska K
Journal of leukocyte biology 2018 Feb;103(2):321-339
Journal of leukocyte biology 2018 Feb;103(2):321-339
Clonally diverse CD38(+)HLA-DR(+)CD8(+) T cells persist during fatal H7N9 disease.
Wang Z, Zhu L, Nguyen THO, Wan Y, Sant S, Quiñones-Parra SM, Crawford JC, Eltahla AA, Rizzetto S, Bull RA, Qiu C, Koutsakos M, Clemens EB, Loh L, Chen T, Liu L, Cao P, Ren Y, Kedzierski L, Kotsimbos T, McCaw JM, La Gruta NL, Turner SJ, Cheng AC, Luciani F, Zhang X, Doherty PC, Thomas PG, Xu J, Kedzierska K
Nature communications 2018 Feb 26;9(1):824
Nature communications 2018 Feb 26;9(1):824
Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.
Van Caeneghem Y, De Munter S, Tieppo P, Goetgeluk G, Weening K, Verstichel G, Bonte S, Taghon T, Leclercq G, Kerre T, Debets R, Vermijlen D, Abken H, Vandekerckhove B
Oncoimmunology 2017;6(3):e1283460
Oncoimmunology 2017;6(3):e1283460
Maintenance of the EBV-specific CD8(+) TCRαβ repertoire in immunosuppressed lung transplant recipients.
Nguyen TH, Bird NL, Grant EJ, Miles JJ, Thomas PG, Kotsimbos TC, Mifsud NA, Kedzierska K
Immunology and cell biology 2017 Jan;95(1):77-86
Immunology and cell biology 2017 Jan;95(1):77-86
High-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine.
O'Connor D, Clutterbuck EA, Thompson AJ, Snape MD, Ramasamy MN, Kelly DF, Pollard AJ
Genome medicine 2017 Jan 30;9(1):11
Genome medicine 2017 Jan 30;9(1):11
IL-15 enhances the anti-tumor activity of trastuzumab against breast cancer cells but causes fatal side effects in humanized tumor mice (HTM).
Wege AK, Weber F, Kroemer A, Ortmann O, Nimmerjahn F, Brockhoff G
Oncotarget 2017 Jan 10;8(2):2731-2744
Oncotarget 2017 Jan 10;8(2):2731-2744
Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.
Kong F, Feng B, Zhang H, Rao H, Wang J, Cong X, Wei L
Experimental and therapeutic medicine 2017 Aug;14(2):1846-1852
Experimental and therapeutic medicine 2017 Aug;14(2):1846-1852
Reduced expression of CD27 by collagenase treatment: implications for interpreting b cell data in tissues.
Shen C, Xu H, Alvarez X, Lackner AA, Veazey RS, Wang X
PloS one 2015;10(3):e0116667
PloS one 2015;10(3):e0116667
Rapid Development of gp120-Focused Neutralizing B Cell Responses during Acute Simian Immunodeficiency Virus Infection of African Green Monkeys.
Amos JD, Himes JE, Armand L, Gurley TC, Martinez DR, Colvin L, Beck K, Overman RG, Liao HX, Moody MA, Permar SR
Journal of virology 2015 Sep;89(18):9485-98
Journal of virology 2015 Sep;89(18):9485-98
Restricted isotype, distinct variable gene usage, and high rate of gp120 specificity of HIV-1 envelope-specific B cells in colostrum compared with those in blood of HIV-1-infected, lactating African women.
Sacha CR, Vandergrift N, Jeffries TL Jr, McGuire E, Fouda GG, Liebl B, Marshall DJ, Gurley TC, Stiegel L, Whitesides JF, Friedman J, Badiabo A, Foulger A, Yates NL, Tomaras GD, Kepler TB, Liao HX, Haynes BF, Moody MA, Permar SR
Mucosal immunology 2015 Mar;8(2):316-26
Mucosal immunology 2015 Mar;8(2):316-26
Phenotypic, genomic and functional characterization reveals no differences between CD138++ and CD138low subpopulations in multiple myeloma cell lines.
Paíno T, Sarasquete ME, Paiva B, Krzeminski P, San-Segundo L, Corchete LA, Redondo A, Garayoa M, García-Sanz R, Gutiérrez NC, Ocio EM, San-Miguel JF
PloS one 2014;9(3):e92378
PloS one 2014;9(3):e92378
Adipose tissue NK cells manifest an activated phenotype in human obesity.
O'Rourke RW, Gaston GD, Meyer KA, White AE, Marks DL
Metabolism: clinical and experimental 2013 Nov;62(11):1557-61
Metabolism: clinical and experimental 2013 Nov;62(11):1557-61
Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.
Ndungu FM, Lundblom K, Rono J, Illingworth J, Eriksson S, Färnert A
European journal of immunology 2013 Nov;43(11):2919-29
European journal of immunology 2013 Nov;43(11):2919-29
Long-term immune reconstitution of naive and memory t cell pools after haploidentical hematopoietic stem cell transplantation.
Azevedo RI, Soares MV, Albuquerque AS, Tendeiro R, Soares RS, Martins M, Ligeiro D, Victorino RM, Lacerda JF, Sousa AE
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2013 May;19(5):703-12
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2013 May;19(5):703-12
Rabbit antithymocyte globulin (thymoglobulin) impairs the thymic output of both conventional and regulatory CD4+ T cells after allogeneic hematopoietic stem cell transplantation in adult patients.
Na IK, Wittenbecher F, Dziubianau M, Herholz A, Mensen A, Kunkel D, Blau O, Blau I, Thiel E, Uharek L, Scheibenbogen C, Rieger K, Thiel A
Haematologica 2013 Jan;98(1):23-30
Haematologica 2013 Jan;98(1):23-30
Follicular helper T cells serve as the major CD4 T cell compartment for HIV-1 infection, replication, and production.
Perreau M, Savoye AL, De Crignis E, Corpataux JM, Cubas R, Haddad EK, De Leval L, Graziosi C, Pantaleo G
The Journal of experimental medicine 2013 Jan 14;210(1):143-56
The Journal of experimental medicine 2013 Jan 14;210(1):143-56
Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.
Illingworth J, Butler NS, Roetynck S, Mwacharo J, Pierce SK, Bejon P, Crompton PD, Marsh K, Ndungu FM
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
B cell analysis of ethnic groups in Mali with differential susceptibility to malaria.
Portugal S, Doumtabe D, Traore B, Miller LH, Troye-Blomberg M, Doumbo OK, Dolo A, Pierce SK, Crompton PD
Malaria journal 2012 May 11;11:162
Malaria journal 2012 May 11;11:162
Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.
Kuzin I, Sun H, Moshkani S, Feng C, Mantalaris A, Wu JH, Bottaro A
Biotechnology and bioengineering 2011 Jun;108(6):1430-40
Biotechnology and bioengineering 2011 Jun;108(6):1430-40
Vaccine-elicited SIV and HIV envelope-specific IgA and IgG memory B cells in rhesus macaque peripheral blood correlate with functional antibody responses and reduced viremia.
Brocca-Cofano E, McKinnon K, Demberg T, Venzon D, Hidajat R, Xiao P, Daltabuit-Test M, Patterson LJ, Robert-Guroff M
Vaccine 2011 Apr 12;29(17):3310-9
Vaccine 2011 Apr 12;29(17):3310-9
Chronic lymphocytic leukaemia (CLL) and CLL-type monoclonal B-cell lymphocytosis (MBL) show differential expression of molecules involved in lymphoid tissue homing.
Rawstron AC, Shingles J, de Tute R, Bennett F, Jack AS, Hillmen P
Cytometry. Part B, Clinical cytometry 2010;78 Suppl 1:S42-6
Cytometry. Part B, Clinical cytometry 2010;78 Suppl 1:S42-6
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Supportive validation
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- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (open histogram) or Anti-Human CD27 PE-Cyanine7 (filled histogram). Cells in the lymphocyte gate were used for analysis.
Supportive validation
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- Figure 4 Early detection of TRAV5-biased GLC-specific CD8 + T cells in patient Tx101. PBMC from patient Tx101 ( a ) at 87 days post transplant underwent tetramer-associated magnetic enrichment (TAME). Pre-enriched, post-enriched and the flowthrough fractions were collected and analyzed based on GLC-tetramer and anti-CD8 antibody staining. Percentages are based on CD3 + T cells. CD3 + T cells, GLC-tetramer + CD8 + and GLC-tetramer + CD8 low/- cells from the post-enriched fraction ( b ) were phenotypically analyzed based on CD45RA and CD27 expression. Pie charts of paired GLC-specific CD8 + TCRalphabeta clonotypes following TAME (d87) ( c ) or following in vitro expansion (d185, d299 and d472) ( d ) are shown. Pie chart area shaded in red represent TRAV5 clonotypes. Percentage of TCRalphabeta clonotypes shared compared to TAME (d87) (bar graph, left y axis) and SDI values (line graph, right y axis) are shown in e . Frequencies of GLC + CD8 + T cells following expansion and clonotype details are listed in f (ND; not determined).
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- Fig. 4 Sanger sequencing validation of multiple V(D)J recombination patterns in single B cells. a Sketching diagram of the single-cell sequencing procedure. We isolated peripheral blood from healthy donors and separated peripheral blood mononuclear cells (PBMCs). Naive B cells (CD19 + CD38 +/- CD10 - CD27 - ), memory B cells (CD19 + CD38 +/- CD10 - CD27 + ), and plasma cells (CD19 + CD38 +++ CD10 - ) were sorted by FACS. The total mRNA in single B cells was reverse transcribed, and the Ig heavy chain and light chain were amplified by multiplex PCR. FACS, fluorescence-activated cell sorting. b Proportions of cells expressing VDJ recombination patterns in single B cells from donor 7, donor 8, and donor 9. c - e Distribution and frequency of IGHV ( c ), IGHD ( d ), and IGHJ ( e ) segments in Ig germline genes in a single cell. f Proportions of single naive B cells, plasma cells, and memory B cells expressing one, two, or three V H DJ H segments. g , h Proportions of single B cells expressing one, two, or more V kappa J kappa segments ( g ) and V lamda J lamda segments ( h ). i Counts of single B cells expressing only Igkappa or Iglamda or expressing both Igkappa and Iglamda. j , k Proportions of naive B cells, plasma cells, and memory B cells expressing one, two, three, or four Ig classes
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- Figure 3 Expression pattern of CD27 and CD28 on gammadelta T cells in AR. (A) Representative flow cytometry plots of the expression of CD27 and CD28 on gammadelta T cells among PBMCs from HC (n=12) and AR (n=14) subjects. (B-G) Quantification of the percentage of (B) CD27+, (C) CD28+, (D) CD27 + CD28 + , (E) CD27 + CD28 - , (F) CD27 - CD28 + and (G) CD27 - CD28 - gammadelta + T cells in PBMCs. Values are expressed as the mean +- standard deviation. AR, allergic rhinitis; HC, healthy controls; PBMCs, peripheral blood mononuclear cells.