Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [13]
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- Product number
- 61-0279-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD27 Monoclonal Antibody (O323), PE-eFluor™ 610, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction. Applications Reported: This O323 antibody has been reported for use in flow cytometric analysis. Applications Tested: This O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- O323
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.
More than one antibody of individual B cells revealed by single-cell immune profiling.
Perturbed CD8(+) T cell immunity across universal influenza epitopes in the elderly.
Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.
Maintenance of the EBV-specific CD8(+) TCRαβ repertoire in immunosuppressed lung transplant recipients.
Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.
Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.
Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.
Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.
Wang Q, Sun Q, Chen Q, Li H, Liu D
Experimental and therapeutic medicine 2020 Dec;20(6):224
Experimental and therapeutic medicine 2020 Dec;20(6):224
More than one antibody of individual B cells revealed by single-cell immune profiling.
Shi Z, Zhang Q, Yan H, Yang Y, Wang P, Zhang Y, Deng Z, Yu M, Zhou W, Wang Q, Yang X, Mo X, Zhang C, Huang J, Dai H, Sun B, Zhao Y, Zhang L, Yang YG, Qiu X
Cell discovery 2019;5:64
Cell discovery 2019;5:64
Perturbed CD8(+) T cell immunity across universal influenza epitopes in the elderly.
Nguyen THO, Sant S, Bird NL, Grant EJ, Clemens EB, Koutsakos M, Valkenburg SA, Gras S, Lappas M, Jaworowski A, Crowe J, Loh L, Kedzierska K
Journal of leukocyte biology 2018 Feb;103(2):321-339
Journal of leukocyte biology 2018 Feb;103(2):321-339
Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.
Van Caeneghem Y, De Munter S, Tieppo P, Goetgeluk G, Weening K, Verstichel G, Bonte S, Taghon T, Leclercq G, Kerre T, Debets R, Vermijlen D, Abken H, Vandekerckhove B
Oncoimmunology 2017;6(3):e1283460
Oncoimmunology 2017;6(3):e1283460
Maintenance of the EBV-specific CD8(+) TCRαβ repertoire in immunosuppressed lung transplant recipients.
Nguyen TH, Bird NL, Grant EJ, Miles JJ, Thomas PG, Kotsimbos TC, Mifsud NA, Kedzierska K
Immunology and cell biology 2017 Jan;95(1):77-86
Immunology and cell biology 2017 Jan;95(1):77-86
Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.
Kong F, Feng B, Zhang H, Rao H, Wang J, Cong X, Wei L
Experimental and therapeutic medicine 2017 Aug;14(2):1846-1852
Experimental and therapeutic medicine 2017 Aug;14(2):1846-1852
Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.
Ndungu FM, Lundblom K, Rono J, Illingworth J, Eriksson S, Färnert A
European journal of immunology 2013 Nov;43(11):2919-29
European journal of immunology 2013 Nov;43(11):2919-29
Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.
Illingworth J, Butler NS, Roetynck S, Mwacharo J, Pierce SK, Bejon P, Crompton PD, Marsh K, Ndungu FM
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.
Kuzin I, Sun H, Moshkani S, Feng C, Mantalaris A, Wu JH, Bottaro A
Biotechnology and bioengineering 2011 Jun;108(6):1430-40
Biotechnology and bioengineering 2011 Jun;108(6):1430-40
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-eFluor® 610 (Product # 61-4714-82) (blue histogram) or Anti-Human CD27 PE-eFluor® 610 (purple histogram). Cells in the lymphocyte gate were used for analysis.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Figure 4 Early detection of TRAV5-biased GLC-specific CD8 + T cells in patient Tx101. PBMC from patient Tx101 ( a ) at 87 days post transplant underwent tetramer-associated magnetic enrichment (TAME). Pre-enriched, post-enriched and the flowthrough fractions were collected and analyzed based on GLC-tetramer and anti-CD8 antibody staining. Percentages are based on CD3 + T cells. CD3 + T cells, GLC-tetramer + CD8 + and GLC-tetramer + CD8 low/- cells from the post-enriched fraction ( b ) were phenotypically analyzed based on CD45RA and CD27 expression. Pie charts of paired GLC-specific CD8 + TCRalphabeta clonotypes following TAME (d87) ( c ) or following in vitro expansion (d185, d299 and d472) ( d ) are shown. Pie chart area shaded in red represent TRAV5 clonotypes. Percentage of TCRalphabeta clonotypes shared compared to TAME (d87) (bar graph, left y axis) and SDI values (line graph, right y axis) are shown in e . Frequencies of GLC + CD8 + T cells following expansion and clonotype details are listed in f (ND; not determined).
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- Experimental details
- Fig. 4 Sanger sequencing validation of multiple V(D)J recombination patterns in single B cells. a Sketching diagram of the single-cell sequencing procedure. We isolated peripheral blood from healthy donors and separated peripheral blood mononuclear cells (PBMCs). Naive B cells (CD19 + CD38 +/- CD10 - CD27 - ), memory B cells (CD19 + CD38 +/- CD10 - CD27 + ), and plasma cells (CD19 + CD38 +++ CD10 - ) were sorted by FACS. The total mRNA in single B cells was reverse transcribed, and the Ig heavy chain and light chain were amplified by multiplex PCR. FACS, fluorescence-activated cell sorting. b Proportions of cells expressing VDJ recombination patterns in single B cells from donor 7, donor 8, and donor 9. c - e Distribution and frequency of IGHV ( c ), IGHD ( d ), and IGHJ ( e ) segments in Ig germline genes in a single cell. f Proportions of single naive B cells, plasma cells, and memory B cells expressing one, two, or three V H DJ H segments. g , h Proportions of single B cells expressing one, two, or more V kappa J kappa segments ( g ) and V lamda J lamda segments ( h ). i Counts of single B cells expressing only Igkappa or Iglamda or expressing both Igkappa and Iglamda. j , k Proportions of naive B cells, plasma cells, and memory B cells expressing one, two, three, or four Ig classes
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- Figure 3 Expression pattern of CD27 and CD28 on gammadelta T cells in AR. (A) Representative flow cytometry plots of the expression of CD27 and CD28 on gammadelta T cells among PBMCs from HC (n=12) and AR (n=14) subjects. (B-G) Quantification of the percentage of (B) CD27+, (C) CD28+, (D) CD27 + CD28 + , (E) CD27 + CD28 - , (F) CD27 - CD28 + and (G) CD27 - CD28 - gammadelta + T cells in PBMCs. Values are expressed as the mean +- standard deviation. AR, allergic rhinitis; HC, healthy controls; PBMCs, peripheral blood mononuclear cells.