Antibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
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- Product number
- NBP2-75198 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal CD27/TNFRSF7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This antibody is specific for the extracellular domain of murine CD27, a TNF-receptor superfamily member.
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 mg
- Storage
- Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- ELISA: CD27/TNFRSF7 Antibody (RM27-3E5) [NBP2-75198] - Binding curves of the rabbit IgG chimeric version of the anti-CD27 (Interleukin-2 receptor subunit alpha) antibody RM27-3E5 (NBP2-75198; red line) and isotype control (anti-Fluorescein antibody; blue line) to an ELISA plate coated with CD27-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10 mg/mL to 0.17 ng/ml of NBP2-75198 was used. CD70 (CD27-L) is the ligand for CD27. CD70 Fc-fusion protein was added to a 10mg/mL solution of NBP2-75198 starting at 10mg/mL, and then at 3-fold dilutions down to 0.17ng/mL (red line). A negative control assay using 4-1BB Fc-fusion protein was also performed (yellow line). For signal detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.