ABIN2191899

antibody from antibodies-online

Targeting: CDH5 7B4, CD144

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Functional assay (Recommended by provider)

Antibody Data

Product number

ABIN2191899

Provider

antibodies-online

Product name

anti-Cadherin 5 (CDH5) antibody (FITC)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Antibody clone number

BV9

Vial size

100 μg

Storage

Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References

Submitted references

VE-Cadherin-mediated cell-cell interaction suppresses sprouting via signaling to MLC2 phosphorylation.

Abraham S, Yeo M, Montero-Balaguer M, Paterson H, Dejana E, Marshall CJ, Mavria G
Current biology : CB 2009 Apr 28;19(8):668-74

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Gas1 is induced by VE-cadherin and vascular endothelial growth factor and inhibits endothelial cell apoptosis.

Spagnuolo R, Corada M, Orsenigo F, Zanetta L, Deuschle U, Sandy P, Schneider C, Drake CJ, Breviario F, Dejana E
Blood 2004 Apr 15;103(8):3005-12

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Vascular trauma induces rapid but transient mobilization of VEGFR2(+)AC133(+) endothelial precursor cells.

Gill M, Dias S, Hattori K, Rivera ML, Hicklin D, Witte L, Girardi L, Yurt R, Himel H, Rafii S
Circulation research 2001 Feb 2;88(2):167-74

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Expression of VE (vascular endothelial)-cadherin and other endothelial-specific markers in haemangiomas.

Martìn-Padura I, De Castellarnau C, Uccini S, Pilozzi E, Natali PG, Nicotra MR, Ughi F, Azzolini C, Dejana E, Ruco L
The Journal of pathology 1995 Jan;175(1):51-7

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Catenin-dependent and -independent functions of vascular endothelial cadherin.

Navarro P, Caveda L, Breviario F, Mândoteanu I, Lampugnani MG, Dejana E
The Journal of biological chemistry 1995 Dec 29;270(52):30965-72

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Functional properties of human vascular endothelial cadherin (7B4/cadherin-5), an endothelium-specific cadherin.

Breviario F, Caveda L, Corada M, Martin-Padura I, Navarro P, Golay J, Introna M, Gulino D, Lampugnani MG, Dejana E
Arteriosclerosis, thrombosis, and vascular biology 1995 Aug;15(8):1229-39

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Figure 1. Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI. Figure 2. HUVEC cells were stained with antibody BV9 in PBS/0.1% saponin for 1h at 4°C.(Black- no stain, Red- is otype control, Blue- HM2032?BV9)

Flow cytometry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Figure 1. Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI. Figure 2. HUVEC cells were stained with antibody BV9 in PBS/0.1% saponin for 1h at 4°C.(Black- no stain, Red- is otype control, Blue- HM2032?BV9)