Antibody data
- Antibody Data
- Antigen structure
- References [30]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF1002 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse VE-Cadherin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse VE-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human VE-Cadherin is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
2208309A
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Role of glutamine synthetase in angiogenesis beyond glutamine synthesis.
CD163+ macrophages promote angiogenesis and vascular permeability accompanied by inflammation in atherosclerosis.
The bone marrow is patrolled by NK cells that are primed and expand in response to systemic viral activation.
Rhodocetin-αβ selectively breaks the endothelial barrier of the tumor vasculature in HT1080 fibrosarcoma and A431 epidermoid carcinoma tumor models.
Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1α.
Niche-mediated depletion of the normal hematopoietic stem cell reservoir by Flt3-ITD-induced myeloproliferation.
Polarized actin and VE-cadherin dynamics regulate junctional remodelling and cell migration during sprouting angiogenesis.
Mutual regulation of tumour vessel normalization and immunostimulatory reprogramming.
Targeting of Mesenchymal Stromal Cells by Cre-Recombinase Transgenes Commonly Used to Target Osteoblast Lineage Cells.
Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes.
Granzyme B Deficiency Protects against Angiotensin II-Induced Cardiac Fibrosis.
HDL activation of endothelial sphingosine-1-phosphate receptor-1 (S1P(1)) promotes regeneration and suppresses fibrosis in the liver.
Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells.
Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases.
Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts.
Blood vessels pattern heparan sulfate gradients between their apical and basolateral aspects.
Neuroligin 1 induces blood vessel maturation by cooperating with the α6 integrin.
Divergent angiocrine signals from vascular niche balance liver regeneration and fibrosis.
Control of retinoid levels by CYP26B1 is important for lymphatic vascular development in the mouse embryo.
Flk1+ and VE-cadherin+ endothelial cells derived from iPSCs recapitulates vascular development during differentiation and display similar angiogenic potential as ESC-derived cells.
Transcriptional targeting of primary and metastatic tumor neovasculature by an adenoviral type 5 roundabout4 vector in mice.
VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd.
Endothelial cells are essential for the self-renewal and repopulation of Notch-dependent hematopoietic stem cells.
Manganese superoxide dismutase expression in endothelial progenitor cells accelerates wound healing in diabetic mice.
Heterozygous deficiency of PHD2 restores tumor oxygenation and inhibits metastasis via endothelial normalization.
Dysfunctional microvasculature as a consequence of shb gene inactivation causes impaired tumor growth.
Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays.
Genetic delivery of the murine equivalent of bevacizumab (avastin), an anti-vascular endothelial growth factor monoclonal antibody, to suppress growth of human tumors in immunodeficient mice.
Effect of gestational nutrition on vascular integrity in the murine placenta.
Lentiviral rescue of vascular endothelial growth factor receptor-2 expression in flk1-/- embryonic stem cells shows early priming of endothelial precursors.
Eelen G, Dubois C, Cantelmo AR, Goveia J, Brüning U, DeRan M, Jarugumilli G, van Rijssel J, Saladino G, Comitani F, Zecchin A, Rocha S, Chen R, Huang H, Vandekeere S, Kalucka J, Lange C, Morales-Rodriguez F, Cruys B, Treps L, Ramer L, Vinckier S, Brepoels K, Wyns S, Souffreau J, Schoonjans L, Lamers WH, Wu Y, Haustraete J, Hofkens J, Liekens S, Cubbon R, Ghesquière B, Dewerchin M, Gervasio FL, Li X, van Buul JD, Wu X, Carmeliet P
Nature 2018 Sep;561(7721):63-69
Nature 2018 Sep;561(7721):63-69
CD163+ macrophages promote angiogenesis and vascular permeability accompanied by inflammation in atherosclerosis.
Guo L, Akahori H, Harari E, Smith SL, Polavarapu R, Karmali V, Otsuka F, Gannon RL, Braumann RE, Dickinson MH, Gupta A, Jenkins AL, Lipinski MJ, Kim J, Chhour P, de Vries PS, Jinnouchi H, Kutys R, Mori H, Kutyna MD, Torii S, Sakamoto A, Choi CU, Cheng Q, Grove ML, Sawan MA, Zhang Y, Cao Y, Kolodgie FD, Cormode DP, Arking DE, Boerwinkle E, Morrison AC, Erdmann J, Sotoodehnia N, Virmani R, Finn AV
The Journal of clinical investigation 2018 Mar 1;128(3):1106-1124
The Journal of clinical investigation 2018 Mar 1;128(3):1106-1124
The bone marrow is patrolled by NK cells that are primed and expand in response to systemic viral activation.
Milo I, Blecher-Gonen R, Barnett-Itzhaki Z, Bar-Ziv R, Tal O, Gurevich I, Feferman T, Drexler I, Amit I, Bousso P, Shakhar G
European journal of immunology 2018 Jul;48(7):1137-1152
European journal of immunology 2018 Jul;48(7):1137-1152
Rhodocetin-αβ selectively breaks the endothelial barrier of the tumor vasculature in HT1080 fibrosarcoma and A431 epidermoid carcinoma tumor models.
Niland S, Komljenovic D, Macas J, Bracht T, Bäuerle T, Liebner S, Eble JA
Oncotarget 2018 Apr 27;9(32):22406-22422
Oncotarget 2018 Apr 27;9(32):22406-22422
Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1α.
Tsang KM, Hyun JS, Cheng KT, Vargas M, Mehta D, Ushio-Fukai M, Zou L, Pajcini KV, Rehman J, Malik AB
Stem cell reports 2017 Sep 12;9(3):796-806
Stem cell reports 2017 Sep 12;9(3):796-806
Niche-mediated depletion of the normal hematopoietic stem cell reservoir by Flt3-ITD-induced myeloproliferation.
Mead AJ, Neo WH, Barkas N, Matsuoka S, Giustacchini A, Facchini R, Thongjuea S, Jamieson L, Booth CAG, Fordham N, Di Genua C, Atkinson D, Chowdhury O, Repapi E, Gray N, Kharazi S, Clark SA, Bouriez T, Woll P, Suda T, Nerlov C, Jacobsen SEW
The Journal of experimental medicine 2017 Jul 3;214(7):2005-2021
The Journal of experimental medicine 2017 Jul 3;214(7):2005-2021
Polarized actin and VE-cadherin dynamics regulate junctional remodelling and cell migration during sprouting angiogenesis.
Cao J, Ehling M, März S, Seebach J, Tarbashevich K, Sixta T, Pitulescu ME, Werner AC, Flach B, Montanez E, Raz E, Adams RH, Schnittler H
Nature communications 2017 Dec 20;8(1):2210
Nature communications 2017 Dec 20;8(1):2210
Mutual regulation of tumour vessel normalization and immunostimulatory reprogramming.
Tian L, Goldstein A, Wang H, Ching Lo H, Sun Kim I, Welte T, Sheng K, Dobrolecki LE, Zhang X, Putluri N, Phung TL, Mani SA, Stossi F, Sreekumar A, Mancini MA, Decker WK, Zong C, Lewis MT, Zhang XH
Nature 2017 Apr 13;544(7649):250-254
Nature 2017 Apr 13;544(7649):250-254
Targeting of Mesenchymal Stromal Cells by Cre-Recombinase Transgenes Commonly Used to Target Osteoblast Lineage Cells.
Zhang J, Link DC
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2016 Nov;31(11):2001-2007
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2016 Nov;31(11):2001-2007
Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes.
Liu Q, Yang R, Huang X, Zhang H, He L, Zhang L, Tian X, Nie Y, Hu S, Yan Y, Zhang L, Qiao Z, Wang QD, Lui KO, Zhou B
Cell research 2016 Jan;26(1):119-30
Cell research 2016 Jan;26(1):119-30
Granzyme B Deficiency Protects against Angiotensin II-Induced Cardiac Fibrosis.
Shen Y, Cheng F, Sharma M, Merkulova Y, Raithatha SA, Parkinson LG, Zhao H, Westendorf K, Bohunek L, Bozin T, Hsu I, Ang LS, Williams SJ, Bleackley RC, Eriksson JE, Seidman MA, McManus BM, Granville DJ
The American journal of pathology 2016 Jan;186(1):87-100
The American journal of pathology 2016 Jan;186(1):87-100
HDL activation of endothelial sphingosine-1-phosphate receptor-1 (S1P(1)) promotes regeneration and suppresses fibrosis in the liver.
Ding BS, Liu CH, Sun Y, Chen Y, Swendeman SL, Jung B, Chavez D, Cao Z, Christoffersen C, Nielsen LB, Schwab SR, Rafii S, Hla T
JCI insight 2016 Dec 22;1(21):e87058
JCI insight 2016 Dec 22;1(21):e87058
Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells.
Chen Q, Zhang H, Liu Y, Adams S, Eilken H, Stehling M, Corada M, Dejana E, Zhou B, Adams RH
Nature communications 2016 Aug 12;7:12422
Nature communications 2016 Aug 12;7:12422
Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases.
Yin H, Frontini MJ, Arpino JM, Nong Z, O'Neil C, Xu Y, Balint B, Ward AD, Chakrabarti S, Ellis CG, Gros R, Pickering JG
The Journal of biological chemistry 2015 Sep 4;290(36):22127-42
The Journal of biological chemistry 2015 Sep 4;290(36):22127-42
Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts.
Zang G, Gustafsson K, Jamalpour M, Hong J, Genové G, Welsh M
BMC cancer 2015 Apr 8;15:234
BMC cancer 2015 Apr 8;15:234
Blood vessels pattern heparan sulfate gradients between their apical and basolateral aspects.
Stoler-Barak L, Moussion C, Shezen E, Hatzav M, Sixt M, Alon R
PloS one 2014;9(1):e85699
PloS one 2014;9(1):e85699
Neuroligin 1 induces blood vessel maturation by cooperating with the α6 integrin.
Samarelli AV, Riccitelli E, Bizzozero L, Silveira TN, Seano G, Pergolizzi M, Vitagliano G, Cascone I, Carpentier G, Bottos A, Primo L, Bussolino F, Arese M
The Journal of biological chemistry 2014 Jul 11;289(28):19466-76
The Journal of biological chemistry 2014 Jul 11;289(28):19466-76
Divergent angiocrine signals from vascular niche balance liver regeneration and fibrosis.
Ding BS, Cao Z, Lis R, Nolan DJ, Guo P, Simons M, Penfold ME, Shido K, Rabbany SY, Rafii S
Nature 2014 Jan 2;505(7481):97-102
Nature 2014 Jan 2;505(7481):97-102
Control of retinoid levels by CYP26B1 is important for lymphatic vascular development in the mouse embryo.
Bowles J, Secker G, Nguyen C, Kazenwadel J, Truong V, Frampton E, Curtis C, Skoczylas R, Davidson TL, Miura N, Hong YK, Koopman P, Harvey NL, François M
Developmental biology 2014 Feb 1;386(1):25-33
Developmental biology 2014 Feb 1;386(1):25-33
Flk1+ and VE-cadherin+ endothelial cells derived from iPSCs recapitulates vascular development during differentiation and display similar angiogenic potential as ESC-derived cells.
Kohler EE, Wary KK, Li F, Chatterjee I, Urao N, Toth PT, Ushio-Fukai M, Rehman J, Park C, Malik AB
PloS one 2013;8(12):e85549
PloS one 2013;8(12):e85549
Transcriptional targeting of primary and metastatic tumor neovasculature by an adenoviral type 5 roundabout4 vector in mice.
Lu ZH, Kaliberov S, Sohn RE, Kaliberova L, Curiel DT, Arbeit JM
PloS one 2013;8(12):e83933
PloS one 2013;8(12):e83933
VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd.
Sun Z, Li X, Massena S, Kutschera S, Padhan N, Gualandi L, Sundvold-Gjerstad V, Gustafsson K, Choy WW, Zang G, Quach M, Jansson L, Phillipson M, Abid MR, Spurkland A, Claesson-Welsh L
The Journal of experimental medicine 2012 Jul 2;209(7):1363-77
The Journal of experimental medicine 2012 Jul 2;209(7):1363-77
Endothelial cells are essential for the self-renewal and repopulation of Notch-dependent hematopoietic stem cells.
Butler JM, Nolan DJ, Vertes EL, Varnum-Finney B, Kobayashi H, Hooper AT, Seandel M, Shido K, White IA, Kobayashi M, Witte L, May C, Shawber C, Kimura Y, Kitajewski J, Rosenwaks Z, Bernstein ID, Rafii S
Cell stem cell 2010 Mar 5;6(3):251-64
Cell stem cell 2010 Mar 5;6(3):251-64
Manganese superoxide dismutase expression in endothelial progenitor cells accelerates wound healing in diabetic mice.
Marrotte EJ, Chen DD, Hakim JS, Chen AF
The Journal of clinical investigation 2010 Dec;120(12):4207-19
The Journal of clinical investigation 2010 Dec;120(12):4207-19
Heterozygous deficiency of PHD2 restores tumor oxygenation and inhibits metastasis via endothelial normalization.
Mazzone M, Dettori D, de Oliveira RL, Loges S, Schmidt T, Jonckx B, Tian YM, Lanahan AA, Pollard P, de Almodovar CR, De Smet F, Vinckier S, Aragonés J, Debackere K, Luttun A, Wyns S, Jordan B, Pisacane A, Gallez B, Lampugnani MG, Dejana E, Simons M, Ratcliffe P, Maxwell P, Carmeliet P
Cell 2009 Mar 6;136(5):839-851
Cell 2009 Mar 6;136(5):839-851
Dysfunctional microvasculature as a consequence of shb gene inactivation causes impaired tumor growth.
Funa NS, Kriz V, Zang G, Calounova G, Akerblom B, Mares J, Larsson E, Sun Y, Betsholtz C, Welsh M
Cancer research 2009 Mar 1;69(5):2141-8
Cancer research 2009 Mar 1;69(5):2141-8
Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays.
Cordey M, Limacher M, Kobel S, Taylor V, Lutolf MP
Stem cells (Dayton, Ohio) 2008 Oct;26(10):2586-94
Stem cells (Dayton, Ohio) 2008 Oct;26(10):2586-94
Genetic delivery of the murine equivalent of bevacizumab (avastin), an anti-vascular endothelial growth factor monoclonal antibody, to suppress growth of human tumors in immunodeficient mice.
Watanabe M, Boyer JL, Hackett NR, Qiu J, Crystal RG
Human gene therapy 2008 Mar;19(3):300-10
Human gene therapy 2008 Mar;19(3):300-10
Effect of gestational nutrition on vascular integrity in the murine placenta.
Rutland CS, Latunde-Dada AO, Thorpe A, Plant R, Langley-Evans S, Leach L
Placenta 2007 Jul;28(7):734-42
Placenta 2007 Jul;28(7):734-42
Lentiviral rescue of vascular endothelial growth factor receptor-2 expression in flk1-/- embryonic stem cells shows early priming of endothelial precursors.
Li X, Edholm D, Lanner F, Breier G, Farnebo F, Dimberg A, Claesson-Welsh L
Stem cells (Dayton, Ohio) 2007 Dec;25(12):2987-95
Stem cells (Dayton, Ohio) 2007 Dec;25(12):2987-95
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse VE-Cadherin by Western Blot. Western blot shows lysates of mouse lung tissue and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse VE-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VE-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse VE-Cadherin by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. Specific bands were detected for VE-Cadherin at approximately 118, 146 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse VE-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- VE-Cadherin in Mouse Heart. VE-Cadherin was detected in immersion fixed paraffin-embedded sections of mouse heart using Goat Anti-Mouse VE-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.