AM26286FC-N

antibody from OriGene

Targeting: CDH5 7B4, CD144

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Functional assay (Data presented on provider website)

Antibody Data

Product number

AM26286FC-N

Provider

OriGene

Product name

VE Cadherin (CDH5) (Extracell. Dom) mouse monoclonal antibody, clone BV9, FITC

Antibody type

Monoclonal

Description

VE Cadherin (CDH5) (Extracell. Dom) mouse monoclonal antibody, clone BV9, FITC

Host

Mouse

Conjugate

Unconjugated

Epitope

CDH5

Isotype

IgG

Antibody clone number

BV9

Vial size

100 µg

Concentration

0.1 mg/ml

References

Submitted references

Nuclear translocation of PKCα isoenzyme is involved in neurogenic commitment of human neural crest-derived periodontal ligament stem cells.

Trubiani O, Guarnieri S, Diomede F, Mariggiò MA, Merciaro I, Morabito C, Cavalcanti MF, Cocco L, Ramazzotti G
Cellular signalling 2016 Nov;28(11):1631-41

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI.

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HUVEC cells were stained with antibody BV9 in PBS/0.1% saponin for 1h at 4°C.(Black- no stain, Red- isotype control, Blue- AM26286PU-N??BV9)

Validation comment

FC