Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 13-1449-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD144 (VE-cadherin) Monoclonal Antibody (16B1), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling. Applications Reported: This 16B1 antibody has been reported for use in flow cytometric analysis, and immunocytochemistry. Applications Tested: This 16B1 antibody has been tested by flow cytometric analysis of Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- 16B1
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Challenges in enumeration of CTCs in breast cancer using techniques independent of cytokeratin expression.
Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.
MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells.
ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells.
Vasculogenic mimicry in small cell lung cancer.
Tumourigenic non-small-cell lung cancer mesenchymal circulating tumour cells: a clinical case study.
Direct conversion of human amniotic cells into endothelial cells without transitioning through a pluripotent state.
Castle J, Morris K, Pritchard S, Kirwan CC
PloS one 2017;12(4):e0175647
PloS one 2017;12(4):e0175647
Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.
Li Y, Brauer PM, Singh J, Xhiku S, Yoganathan K, Zúñiga-Pflücker JC, Anderson MK
Stem cell reports 2017 Sep 12;9(3):779-795
Stem cell reports 2017 Sep 12;9(3):779-795
MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells.
Almalki SG, Llamas Valle Y, Agrawal DK
Stem cells translational medicine 2017 May;6(5):1385-1398
Stem cells translational medicine 2017 May;6(5):1385-1398
ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells.
Almalki SG, Agrawal DK
Stem cell research & therapy 2017 May 12;8(1):113
Stem cell research & therapy 2017 May 12;8(1):113
Vasculogenic mimicry in small cell lung cancer.
Williamson SC, Metcalf RL, Trapani F, Mohan S, Antonello J, Abbott B, Leong HS, Chester CP, Simms N, Polanski R, Nonaka D, Priest L, Fusi A, Carlsson F, Carlsson A, Hendrix MJ, Seftor RE, Seftor EA, Rothwell DG, Hughes A, Hicks J, Miller C, Kuhn P, Brady G, Simpson KL, Blackhall FH, Dive C
Nature communications 2016 Nov 9;7:13322
Nature communications 2016 Nov 9;7:13322
Tumourigenic non-small-cell lung cancer mesenchymal circulating tumour cells: a clinical case study.
Morrow CJ, Trapani F, Metcalf RL, Bertolini G, Hodgkinson CL, Khandelwal G, Kelly P, Galvin M, Carter L, Simpson KL, Williamson S, Wirth C, Simms N, Frankliln L, Frese KK, Rothwell DG, Nonaka D, Miller CJ, Brady G, Blackhall FH, Dive C
Annals of oncology : official journal of the European Society for Medical Oncology 2016 Jun;27(6):1155-60
Annals of oncology : official journal of the European Society for Medical Oncology 2016 Jun;27(6):1155-60
Direct conversion of human amniotic cells into endothelial cells without transitioning through a pluripotent state.
Ginsberg M, Schachterle W, Shido K, Rafii S
Nature protocols 2015 Dec;10(12):1975-85
Nature protocols 2015 Dec;10(12):1975-85
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.25 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or 0.125 µg of Anti-Human CD144 (VE-Cadherin) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
- Conjugate
- Biotin