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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 14-1449-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD144 (VE-cadherin) Monoclonal Antibody (16B1), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling. Applications Reported: This 16B1 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), microscopy, and immunocytochemistry without fixation. Applications Tested: This 16B1 antibody has been tested by immunoblotting Human Umbilical Vein Endothelial Cell (HUVEC) lysates. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 16B1
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Challenges in enumeration of CTCs in breast cancer using techniques independent of cytokeratin expression.
Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.
MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells.
ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells.
Mitochondria-Targeted Antioxidant SkQR1 Reduces TNF-Induced Endothelial Permeability in vitro.
Vasculogenic mimicry in small cell lung cancer.
Definitive Hematopoietic Multipotent Progenitor Cells Are Transiently Generated From Hemogenic Endothelial Cells in Human Pluripotent Stem Cells.
Tumourigenic non-small-cell lung cancer mesenchymal circulating tumour cells: a clinical case study.
Rab11a Mediates Vascular Endothelial-Cadherin Recycling and Controls Endothelial Barrier Function.
Fc gamma receptor IIb participates in maternal IgG trafficking of human placental endothelial cells.
Histone Demethylases KDM4A and KDM4C Regulate Differentiation of Embryonic Stem Cells to Endothelial Cells.
Direct conversion of human amniotic cells into endothelial cells without transitioning through a pluripotent state.
PDGFRα and CD51 mark human nestin+ sphere-forming mesenchymal stem cells capable of hematopoietic progenitor cell expansion.
Neovascular niche for human myeloma cells in immunodeficient mouse bone.
Identification of endothelial cell junctional proteins and lymphocyte receptors involved in transendothelial migration of human effector memory CD4+ T cells.
High harvest yield, high expansion, and phenotype stability of CD146 mesenchymal stromal cells from whole primitive human umbilical cord tissue.
Perioculomotor cell groups in monkey and man defined by their histochemical and functional properties: reappraisal of the Edinger-Westphal nucleus.
Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
Castle J, Morris K, Pritchard S, Kirwan CC
PloS one 2017;12(4):e0175647
PloS one 2017;12(4):e0175647
Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.
Li Y, Brauer PM, Singh J, Xhiku S, Yoganathan K, Zúñiga-Pflücker JC, Anderson MK
Stem cell reports 2017 Sep 12;9(3):779-795
Stem cell reports 2017 Sep 12;9(3):779-795
MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells.
Almalki SG, Llamas Valle Y, Agrawal DK
Stem cells translational medicine 2017 May;6(5):1385-1398
Stem cells translational medicine 2017 May;6(5):1385-1398
ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells.
Almalki SG, Agrawal DK
Stem cell research & therapy 2017 May 12;8(1):113
Stem cell research & therapy 2017 May 12;8(1):113
Mitochondria-Targeted Antioxidant SkQR1 Reduces TNF-Induced Endothelial Permeability in vitro.
Galkin II, Pletjushkina OY, Zinovkin RA, Zakharova VV, Chernyak BV, Popova EN
Biochemistry. Biokhimiia 2016 Oct;81(10):1188-1197
Biochemistry. Biokhimiia 2016 Oct;81(10):1188-1197
Vasculogenic mimicry in small cell lung cancer.
Williamson SC, Metcalf RL, Trapani F, Mohan S, Antonello J, Abbott B, Leong HS, Chester CP, Simms N, Polanski R, Nonaka D, Priest L, Fusi A, Carlsson F, Carlsson A, Hendrix MJ, Seftor RE, Seftor EA, Rothwell DG, Hughes A, Hicks J, Miller C, Kuhn P, Brady G, Simpson KL, Blackhall FH, Dive C
Nature communications 2016 Nov 9;7:13322
Nature communications 2016 Nov 9;7:13322
Definitive Hematopoietic Multipotent Progenitor Cells Are Transiently Generated From Hemogenic Endothelial Cells in Human Pluripotent Stem Cells.
Bai H, Liu Y, Xie Y, Hoyle DL, Brodsky RA, Cheng L, Cheng T, Wang ZZ
Journal of cellular physiology 2016 May;231(5):1065-76
Journal of cellular physiology 2016 May;231(5):1065-76
Tumourigenic non-small-cell lung cancer mesenchymal circulating tumour cells: a clinical case study.
Morrow CJ, Trapani F, Metcalf RL, Bertolini G, Hodgkinson CL, Khandelwal G, Kelly P, Galvin M, Carter L, Simpson KL, Williamson S, Wirth C, Simms N, Frankliln L, Frese KK, Rothwell DG, Nonaka D, Miller CJ, Brady G, Blackhall FH, Dive C
Annals of oncology : official journal of the European Society for Medical Oncology 2016 Jun;27(6):1155-1160
Annals of oncology : official journal of the European Society for Medical Oncology 2016 Jun;27(6):1155-1160
Rab11a Mediates Vascular Endothelial-Cadherin Recycling and Controls Endothelial Barrier Function.
Yan Z, Wang ZG, Segev N, Hu S, Minshall RD, Dull RO, Zhang M, Malik AB, Hu G
Arteriosclerosis, thrombosis, and vascular biology 2016 Feb;36(2):339-49
Arteriosclerosis, thrombosis, and vascular biology 2016 Feb;36(2):339-49
Fc gamma receptor IIb participates in maternal IgG trafficking of human placental endothelial cells.
Ishikawa T, Takizawa T, Iwaki J, Mishima T, Ui-Tei K, Takeshita T, Matsubara S, Takizawa T
International journal of molecular medicine 2015 May;35(5):1273-89
International journal of molecular medicine 2015 May;35(5):1273-89
Histone Demethylases KDM4A and KDM4C Regulate Differentiation of Embryonic Stem Cells to Endothelial Cells.
Wu L, Wary KK, Revskoy S, Gao X, Tsang K, Komarova YA, Rehman J, Malik AB
Stem cell reports 2015 Jul 14;5(1):10-21
Stem cell reports 2015 Jul 14;5(1):10-21
Direct conversion of human amniotic cells into endothelial cells without transitioning through a pluripotent state.
Ginsberg M, Schachterle W, Shido K, Rafii S
Nature protocols 2015 Dec;10(12):1975-85
Nature protocols 2015 Dec;10(12):1975-85
PDGFRα and CD51 mark human nestin+ sphere-forming mesenchymal stem cells capable of hematopoietic progenitor cell expansion.
Pinho S, Lacombe J, Hanoun M, Mizoguchi T, Bruns I, Kunisaki Y, Frenette PS
The Journal of experimental medicine 2013 Jul 1;210(7):1351-67
The Journal of experimental medicine 2013 Jul 1;210(7):1351-67
Neovascular niche for human myeloma cells in immunodeficient mouse bone.
Iriuchishima H, Takubo K, Miyakawa Y, Nakamura-Ishizu A, Miyauchi Y, Fujita N, Miyamoto K, Miyamoto T, Ikeda E, Kizaki M, Nojima Y, Suda T
PloS one 2012;7(2):e30557
PloS one 2012;7(2):e30557
Identification of endothelial cell junctional proteins and lymphocyte receptors involved in transendothelial migration of human effector memory CD4+ T cells.
Manes TD, Pober JS
Journal of immunology (Baltimore, Md. : 1950) 2011 Feb 1;186(3):1763-8
Journal of immunology (Baltimore, Md. : 1950) 2011 Feb 1;186(3):1763-8
High harvest yield, high expansion, and phenotype stability of CD146 mesenchymal stromal cells from whole primitive human umbilical cord tissue.
Schugar RC, Chirieleison SM, Wescoe KE, Schmidt BT, Askew Y, Nance JJ, Evron JM, Peault B, Deasy BM
Journal of biomedicine & biotechnology 2009;2009:789526
Journal of biomedicine & biotechnology 2009;2009:789526
Perioculomotor cell groups in monkey and man defined by their histochemical and functional properties: reappraisal of the Edinger-Westphal nucleus.
Horn AK, Eberhorn A, Härtig W, Ardeleanu P, Messoudi A, Büttner-Ennever JA
The Journal of comparative neurology 2008 Mar 20;507(3):1317-35
The Journal of comparative neurology 2008 Mar 20;507(3):1317-35
Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
Stem cells (Dayton, Ohio) 2007 Feb;25(2):490-9
Stem cells (Dayton, Ohio) 2007 Feb;25(2):490-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were resolved by SDS -PAGE and immunoblotted with 2 µg/mL of Anti-Human CD144 (VE-Cadherin) Purified and revealed with Anti-Rat IgG HRP.
