A02632-1

antibody from Boster Biological Technology

Targeting: CDH5 7B4, CD144

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A02632-1

Provider

Boster Biological Technology

Product name

Anti-VE-Cadherin-5 CDH5-Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for VE Cadherin detection. Tested with WB, IHC-P, IHC-F, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

The Regulatory Effect of VEGF-Ax on Rat Bone Marrow Mesenchymal Stem Cells' Angioblastic Differentiation and Its Proangiogenic Ability.

Li J, Li Z, Wang C, Li Z, Xu H, Hu Y, Tan Z, Zhang F, Liu C, Yang M, Wang Y, Jin Y, Peng Z, Biswas S, Zhu L
Stem cells and development 2020 May 15;29(10):667-677

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Effect of grape seed proanthocyanidins on tumor vasculogenic mimicry in human triple-negative breast cancer cells.

Luan YY, Liu ZM, Zhong JY, Yao RY, Yu HS
Asian Pacific journal of cancer prevention : APJCP 2015;16(2):531-5

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of VE Cadherin using anti-VE Cadherin antibody (A02632-1). VE Cadherin was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-VE Cadherin Antibody (A02632-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image