A02632-2

antibody from Boster Biological Technology

Targeting: CDH5 7B4, CD144

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A02632-2

Provider

Boster Biological Technology

Product name

Anti-VE-Cadherin-5 Cdh5-Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for VE Cadherin detection. Tested with WB, IHC-P, ELISA(Cap) in Mouse;Rat.

Reactivity

Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Dihydrocapsaicin suppresses the STING-mediated accumulation of ROS and NLRP3 inflammasome and alleviates apoptosis after ischemia-reperfusion injury of perforator skin flap.

Lai Y, Yang N, Chen X, Ma X, Chen Z, Dong C, Yu G, Huang Y, Shi D, Fang P, Fu K, Jiang R, Mao C, Ding J, Gao W
Phytotherapy research : PTR 2024 May;38(5):2539-2559

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Celastrol enhances the viability of random-pattern skin flaps by regulating autophagy through the AMPK-mTOR-TFEB axis.

Gu M, Li C, Deng Q, Chen X, Lei R
Phytotherapy research : PTR 2024 Jun;38(6):3020-3036

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Rosuvastatin promotes survival of random skin flaps through AMPK-mTOR pathway-induced autophagy.

Ye H, Li F, Shen Y, Wu X, Zhao L, Zhang H, Yang J, Shui X
International immunopharmacology 2023 May;118:110059

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Protein kinase D1 promotes the survival of random-pattern skin flaps in rats.

Chen J, Chen H, Muhammad I, Han T, Zhang D, Li B, Zhou X, Zhou F
Biochemical and biophysical research communications 2023 Jan 22;641:67-76

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Effects of limonin treatment on the survival of random skin flaps in mice.

Zhang T, Huang Q, Gan K, Zhou K, Hu K, Ding W, Jin J, Li J
Frontiers in surgery 2022;9:1043239

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Catalpol Enhances Random-Pattern Skin Flap Survival by Activating SIRT1-Mediated Enhancement of Autophagy.

Jiang RH, Dong CJ, Chen ZL, Cheng S, Yang JX, Gao WY
Oxidative medicine and cellular longevity 2022;2022:5668226

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Targeting TFE3 Protects Against Lysosomal Malfunction-Induced Pyroptosis in Random Skin Flaps via ROS Elimination.

Li J, Lou J, Yu G, Chen Y, Chen R, Chen Z, Wu C, Ding J, Xu Y, Jiang J, Xu H, Zhu X, Gao W, Zhou K
Frontiers in cell and developmental biology 2021;9:643996

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Pseudoginsenoside F11 Enhances the Viability of Random-Pattern Skin Flaps by Promoting TFEB Nuclear Translocation Through AMPK-mTOR Signal Pathway.

Zhou F, Zhang X, Jiang L, Li S, Chen Y, Wu J
Frontiers in pharmacology 2021;12:667524

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Exenatide improves random-pattern skin flap survival via TFE3 mediated autophagy augment.

Li J, Chen H, Lou J, Bao G, Wu C, Lou Z, Wang X, Ding J, Li Z, Xiao J, Xu H, Gao W, Zhou K
Journal of cellular physiology 2021 May;236(5):3641-3659

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Hydroxysafflor yellow A promotes multiterritory perforating flap survival: an experimental study.

Zhang C, Shao Z, Chen Z, Lin C, Hu S, Lou Z, Li J, Zheng X, Lin N, Gao W
American journal of translational research 2020;12(8):4781-4794

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Detrimental Effect of Sitagliptin Induced Autophagy on Multiterritory Perforator Flap Survival.

Chen Z, Zhang C, Ma H, Huang Z, Li J, Lou J, Li B, Tu Q, Gao W
Frontiers in pharmacology 2020;11:951

---

Butylphthalide Inhibits Autophagy and Promotes Multiterritory Perforator Flap Survival.

Li B, Chen Z, Luo X, Zhang C, Chen H, Wang S, Zhao M, Ma H, Liu J, Cheng M, Yang Y, Yan H
Frontiers in pharmacology 2020;11:612932

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miR-181b-5p inhibits endothelial-mesenchymal transition in monocrotaline-induced pulmonary arterial hypertension by targeting endocan and TGFBR1.

Zhao H, Wang Y, Zhang X, Guo Y, Wang X
Toxicology and applied pharmacology 2020 Jan 1;386:114827

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Metformin Promotes the Survival of Random-Pattern Skin Flaps by Inducing Autophagy via the AMPK-mTOR-TFEB signaling pathway.

Wu H, Ding J, Li S, Lin J, Jiang R, Lin C, Dai L, Xie C, Lin D, Xu H, Gao W, Zhou K
International journal of biological sciences 2019;15(2):325-340

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Therapeutic potential of pravastatin for random skin flaps necrosis: involvement of promoting angiogenesis and inhibiting apoptosis and oxidative stress.

Lin J, Jia C, Wang Y, Jiang S, Jia Z, Chen N, Sheng S, Li S, Jiang L, Xu H, Zhou K, Chen Y
Drug design, development and therapy 2019;13:1461-1472

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Betulinic Acid Enhances the Viability of Random-Pattern Skin Flaps by Activating Autophagy.

Li J, Bao G, ALyafeai E, Ding J, Li S, Sheng S, Shen Z, Jia Z, Lin C, Zhang C, Lou Z, Xu H, Gao W, Zhou K
Frontiers in pharmacology 2019;10:1017

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FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival.

Zhou K, Chen H, Lin J, Xu H, Wu H, Bao G, Li J, Deng X, Shui X, Gao W, Ding J, Xiao J, Xu H
Cell death & disease 2019 Nov 18;10(12):872

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Salvianolic Acid B Promotes the Survival of Random-Pattern Skin Flaps in Rats by Inducing Autophagy.

Lin J, Lin R, Li S, Wu H, Ding J, Xiang G, Li S, Wang Y, Lin D, Gao W, Kong J, Xu H, Zhou K
Frontiers in pharmacology 2018;9:1178

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Exercise Mitigates Alcohol Induced Endoplasmic Reticulum Stress Mediated Cognitive Impairment through ATF6-Herp Signaling.

George AK, Behera J, Kelly KE, Mondal NK, Richardson KP, Tyagi N
Scientific reports 2018 Mar 26;8(1):5158

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of VE Cadherin using anti-VE Cadherin antibody (A02632-2). VE Cadherin was detected in paraffin-embedded section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-VE Cadherin Antibody (A02632-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image