PA5-17401

antibody from Invitrogen Antibodies

Targeting: CDH5 7B4, CD144

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17401

Provider

Invitrogen Antibodies

Product name

VE-cadherin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Concentration

22 µg/mL

References

Submitted references

FTY720 Protects Against Ischemia-Reperfusion Injury by Preventing the Redistribution of Tight Junction Proteins and Decreases Inflammation in the Subacute Phase in an Experimental Stroke Model.

Wang Z, Higashikawa K, Yasui H, Kuge Y, Ohno Y, Kihara A, Midori YA, Houkin K, Kawabori M
Translational stroke research 2020 Oct;11(5):1103-1116

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Tracking adiponectin biodistribution via fluorescence molecular tomography indicates increased vascular permeability after streptozotocin-induced diabetes.

Yoon N, Dadson K, Dang T, Chu T, Noskovicova N, Hinz B, Raignault A, Thorin E, Kim S, Jeon JS, Jonkman J, McKee TD, Grant J, Peterson JD, Kelly SP, Sweeney G
American journal of physiology. Endocrinology and metabolism 2019 Nov 1;317(5):E760-E772

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Extracellular S100A4 affects endothelial cell integrity and stimulates transmigration of A375 melanoma cells.

Herwig N, Belter B, Pietzsch J
Biochemical and biophysical research communications 2016 Sep 2;477(4):963-969

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of VE-Cadherin in extracts from HUVEC and BAEC cells using VE-Cadherin polyclonal antibody (Product # PA5-17401).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of VE-cadherin was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VE-cadherin Polyclonal Antibody (Product # PA5-17401) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic punctas and plasma membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of VE-Cadherin in HUVE cells using a VE-Cadherin polyclonal antibody (Product # PA5-17401) (green) and a MEK1/2 monoclonal antibody (red). DNA is labeled using a fluorescent blue dye.