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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Western blot [1]
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- Product number
- NBP1-97512 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-97512, RRID:AB_11190601
- Product name
- Rat Monoclonal Perforin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Recognizes mouse perforin (region aa 402-534).
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Antiviral CD8 T cells induce Zika-virus-associated paralysis in mice.
ADP-ribose/TRPM2-mediated Ca2+ signaling is essential for cytolytic degranulation and antitumor activity of natural killer cells.
Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells.
Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells.
Cubilin, a binding partner for galectin-3 in the murine utero-placental complex.
Resistance of CTL to perforin-mediated lysis. Evidence for a lymphocyte membrane protein interacting with perforin.
The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes.
The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes.
Jurado KA, Yockey LJ, Wong PW, Lee S, Huttner AJ, Iwasaki A
Nature microbiology 2018 Feb;3(2):141-147
Nature microbiology 2018 Feb;3(2):141-147
ADP-ribose/TRPM2-mediated Ca2+ signaling is essential for cytolytic degranulation and antitumor activity of natural killer cells.
Rah SY, Kwak JY, Chung YJ, Kim UH
Scientific reports 2015 Mar 25;5:9482
Scientific reports 2015 Mar 25;5:9482
Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells.
Dalton JE, Howell G, Pearson J, Scott P, Carding SR
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3660-7
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3660-7
Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells.
Dalton JE, Howell G, Pearson J, Scott P, Carding SR
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3660-7
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3660-7
Cubilin, a binding partner for galectin-3 in the murine utero-placental complex.
Crider-Pirkle S, Billingsley P, Faust C, Hardy DM, Lee V, Weitlauf H
The Journal of biological chemistry 2002 May 3;277(18):15904-12
The Journal of biological chemistry 2002 May 3;277(18):15904-12
Resistance of CTL to perforin-mediated lysis. Evidence for a lymphocyte membrane protein interacting with perforin.
Müller C, Tschopp J
Journal of immunology (Baltimore, Md. : 1950) 1994 Sep 15;153(6):2470-8
Journal of immunology (Baltimore, Md. : 1950) 1994 Sep 15;153(6):2470-8
The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes.
Dupuis M, Schaerer E, Krause KH, Tschopp J
The Journal of experimental medicine 1993 Jan 1;177(1):1-7
The Journal of experimental medicine 1993 Jan 1;177(1):1-7
The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes.
Dupuis M, Schaerer E, Krause KH, Tschopp J
The Journal of experimental medicine 1993 Jan 1;177(1):1-7
The Journal of experimental medicine 1993 Jan 1;177(1):1-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Perforin Antibody (CB5.4) [NBP1-97512] - Detection of perforin in the mouse T cell clone B6.1. (lane 1). Absence of perforin in the mouse fibroblast cell line NIH.3T3 (lane 2). The protein migrates as a 66 kDa species. Method: Cell extracts from the T cell clone B6.1 (2x10^6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.
