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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP1-97440 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-97440, RRID:AB_11189800
- Product name
- Mouse Monoclonal Perforin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Recognizes an epitope in the region aa 402-534 of human and mouse perforin. Does NOT detect Human in Western Blot.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Perforin Antibody (CE2.10) [NBP1-97440] - Detectioon of perforin in the mouse T cell clone B6.1. (lane 2). As control cell line HEK 293 was used (lane 1). The protein migrates around ~66kDa.Method: Cell extracts from the T cell clone B6.1 (2x10^6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CE2.10. antibody at 1ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.
