308122

antibody from BioLegend

Targeting: PRF1 HPLH2, P1, PFP

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

308122

Provider

BioLegend

Proper citation

BioLegend Cat#308122, RRID:AB_2566204

Product name

Brilliant Violet 421™ anti-human Perforin

Antibody type

Monoclonal

Antigen

Purified granules from the human lymphoma cell line

Description

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Reactivity

Human, Bovine, Feline

Host

Mouse

Conjugate

Blue dye

Antibody clone number

dG9

Vial size

100 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes were surface stained with CD8 APC, fixed with Fixation Buffer, permeabilized with Permeabilization Wash Buffer, and then intracellularly stained with perforin (clone dG9) Brilliant Violet 421™ (top), or mouse IgG2b, κ Brilliant Violet 421™ isotype control (bottom).

Conjugate

Blue dye