308124

antibody from BioLegend

Targeting: PRF1 HPLH2, P1, PFP

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

308124

Provider

BioLegend

Proper citation

BioLegend Cat#308124, RRID:AB_2566802

Product name

Alexa Fluor® 594 anti-human Perforin

Antibody type

Monoclonal

Antigen

Purified granules from the human lymphoma cell line

Reactivity

Human, Bovine, Feline

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

dG9

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes at room temperature. Then the cells were stained with 5 µg/ml Perforin (clone dG9) Alexa Fluor® 594 (red) and CD8 (clone HIT8a) Alexa Fluor® 488 (green) for one hour. The nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.

Conjugate

Yellow dye