Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 308124 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#308124, RRID:AB_2566802
- Product name
- Alexa Fluor® 594 anti-human Perforin
- Antibody type
- Monoclonal
- Antigen
- Purified granules from the human lymphoma cell line
- Reactivity
- Human, Bovine, Feline
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- dG9
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes at room temperature. Then the cells were stained with 5 µg/ml Perforin (clone dG9) Alexa Fluor® 594 (red) and CD8 (clone HIT8a) Alexa Fluor® 488 (green) for one hour. The nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
- Conjugate
- Yellow dye