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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00005551-M04 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005551-M04, RRID:AB_1579098
- Product name
- PRF1 monoclonal antibody (M04), clone 3B4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant PRF1.
- Antigen sequence
WSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLG
TCDQAPKSGSHEVRCNLNHGHLKFRYHARCLPHLG
GGTCLDYVPQMLLGEPPGNRSGAVW- Isotype
- IgG
- Antibody clone number
- 3B4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PRF1 expression in transfected 293T cell line by PRF1 monoclonal antibody (M04), clone 3B4.Lane 1: PRF1 transfected lysate(61.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PRF1 is 0.1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PRF1 transfected lysate using anti-PRF1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PRF1 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
