Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations [0]
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- Product number
- 14-9994-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Perforin Monoclonal Antibody (dG9 (delta G9)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections. Applications Reported: This dG9 (delta G9) antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. (Fluorochrome conjugated dG9 (delta G9) is recommended for use in intracellular flow cytometry.). Applications Tested: This dG9 (delta G9) antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- dG9 (delta G9)
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Functional dichotomy of Vδ2 γδ T cells in chronic hepatitis C virus infections: role in cytotoxicity but not for IFN-γ production.
Insufficient disease inhibition by intrathecal rituximab in progressive multiple sclerosis.
Lipoxin A4 regulates natural killer cell and type 2 innate lymphoid cell activation in asthma.
Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody.
Yin W, Tong S, Zhang Q, Shao J, Liu Q, Peng H, Hu H, Peng M, Hu P, Ren H, Tian Z, Zhang D
Scientific reports 2016 May 19;6:26296
Scientific reports 2016 May 19;6:26296
Insufficient disease inhibition by intrathecal rituximab in progressive multiple sclerosis.
Komori M, Lin YC, Cortese I, Blake A, Ohayon J, Cherup J, Maric D, Kosa P, Wu T, Bielekova B
Annals of clinical and translational neurology 2016 Mar;3(3):166-79
Annals of clinical and translational neurology 2016 Mar;3(3):166-79
Lipoxin A4 regulates natural killer cell and type 2 innate lymphoid cell activation in asthma.
Barnig C, Cernadas M, Dutile S, Liu X, Perrella MA, Kazani S, Wechsler ME, Israel E, Levy BD
Science translational medicine 2013 Feb 27;5(174):174ra26
Science translational medicine 2013 Feb 27;5(174):174ra26
Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody.
Hameed A, Olsen KJ, Cheng L, Fox WM 3rd, Hruban RH, Podack ER
The American journal of pathology 1992 May;140(5):1025-30
The American journal of pathology 1992 May;140(5):1025-30
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